桦褐孔菌提取物抗氧化活性研究

黄纪国<sup>1; 2</sup>; 余雄涛<sup>1; 2</sup>; 谢意珍<sup>1; 2</sup>; 潘鸿辉<sup>1; 2*</sup>; 蓝宇虹<sup>1</sup>; 吕 静<sup>2</sup>

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桦褐孔菌提取物抗氧化活性研究
黄纪国^1,2, 余雄涛^1,2, 谢意珍^1,2, 潘鸿辉^1,2*, 蓝宇虹¹, 吕静²
1. 广东省微生物研究所, 省部共建华南应用微生物国家重点实验室, 广州 510070;2. 广东粤微食用菌技术有限公司, 广州 510663

摘要:

以乙酸乙酯和甲醇为提取剂,采用索氏提取法,剩余残渣采用热水浸提,最终得到桦褐孔菌不同极性提取物,对其DPPH自由基、羟基自由基及超氧阴离子自由基清除活性作用进行了研究,确定桦褐孔菌的抗氧化能力,为深入研究和开发桦褐孔菌功能性食品奠定理论基础。实验结果表明桦褐孔菌具有较好的抗氧化活性,其中乙酸乙酯提取物的DPPH自由基清除率、羟基自由基清除率和超氧阴离子自由基清除率均高于其他两组分及BHT,桦褐孔菌提取物有望成为功能性食品组分中合成抗氧化剂的天然替代品。

关键词: 桦褐孔菌抗氧化 DPPH

DOI：10.3969/j.issn.1000-3142.2014.04.017

分类号:S646

Fund project:

Study on antioxidant activity from Inonotus obliquus

HUANG Ji-Guo^1,2, YU Xiong-Tao^1,2, XIE Yi-Zhen^1,2, PAN Hong-Hui^1,2*, LAN Yu-Hong¹, LÜ Jing²

1. Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology, South China (The Ministry Province Joint Development), Guangzhou 510070, China;2. Guangdong Yuewei Edible Fungi Technology Co. Ltd, Guangzhou 510663, China

Abstract:

Inonotus obliquus belongs to the family Hymenochaetaceae of Basidiomycetes,is mainly distributed in Russia,North America,Europe,Japan and northeastern China. It has been used traditionally as a folk medicine and with other herbs to reduce infammation in the nasopharynx and facilitate breathing. Different extracts were based on ethyl acetate(EA),methanol and water as extraction solvent and using Soxhlet extraction method to Inonotus obliquus, respectively. Reducing and radical scavenging abilities against DPPH radica,hydroxyl radical,superoxide radical and reducing power of ethyl acetate(EA),methanol extracts and water extracts were measured by means of photometric method,then compared with BHT. The results showed that ethyl acetate(EA)extract from the fruit of Inonotus obliquus had the highestantioxidant activity,and its antioxidant activity was better than that of BHT. It was correspond with the contents of various kinds of triterpenes,lignins and polyphenols in the different extracts. EA extracts would be good natural antioxidant,and have good development value.

Key words: Inonotus obliquus antioxidant DPPH