| 摘要: |
| 通过静态吸附比较三种大孔树脂对甜菊糖中莱鲍迪甙A(RA)和甜菊甙(ST)的吸附和解吸能力,利用高效液相色谱对结果进行检测,从中筛选出对RA和ST吸附效果最佳的LX-68M树脂。纯化工艺为30 ℃下,LX-68M树脂对RA和ST在6 h后达到吸附饱和,吸附量分别为干树脂RA 85.00 mg/g和ST 121.50 mg/g。动态洗脱结果表明:以55%的乙醇为洗脱液,在2 BV/h的洗脱速度下,经LX-68M树脂处理后的甜菊糖溶液纯度由75.5%提高到77.8%。表明LX-68M树脂对于甜叶菊糖苷工业生产的应用前景较好。 |
| 关键词: 大孔树脂 莱鲍迪甙A 甜菊甙 分离纯化 高效液相色谱 |
| DOI：10.3969/j.issn.1000-3142.2014.04.019 |
| 分类号:TQ461 |
| Fund project: |
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| Purification of stevioside ST and RA by macroporous resin |
|
YANG Xiao-Yu1, CHEN Zhi-Liang2, HAO Zai-Bin2*
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1. Hunan Chemical Industry Professional and Technology College, Zhuzhou 414002, China;2. Chemical and
Biological Engineering College, Guilin University of Technology, Guilin 541004, China
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| Abstract: |
| The adsorption and desorption capacities of three kinds of macroporous resins for RA and ST contained in stevioside were investigated through static adsorption experiment. The adsorption and desorption capacities was quantified using high performance liquid chromatography(HPLC),and the results indicated that the LX-68M had the best adsorption and desorption capacities. Under 30 ℃,the absorption equilibrium of LX-68M resin was achieved for RA(85.0 mg/g dry resin)and ST(121.5 mg/g dry resin)after 6 h,respectively. The dynamic desorption was carried out under 2 BV/h eluting employing 55%(V%)ethanol/water as eluent,and the results showed that the purity of stevia glycosides increased from 75.5% to 77.8%. On the basis of these findings,it was believed that the LX-68M resin had industrial application promise for stevia glycosides. |
| Key words: macroporous resin rebaudioside A stevioside purification HPLC |
