珍稀濒危植物太行菊遗传多样性的ISSR分析

收稿日期: 2013-10-14修回日期: 2013-12-15 基金项目: 焦作市科技计划项目(201204001) 作者简介: 张安世(1965-),男,河南博爱人,教授,主要从事植物分子生物学研究,(E-mail)[e-mail]。*通讯作者 Genetic diversity of the rare and endangered plant Opisthopapus taihangensis detected by ISSR analysis

关键词:  太行菊  ISSR  遗传多样性  遗传分化  保护策略

Key words:  Opisthopapus taihangensis  ISSR  genetic diversity  geneticdifferentiation  conservation strategy

摘要:

利用ISSR分子标记技术对太行山特有濒危物种太行菊11个自然居群的遗传多样性进行研究。用10个引物对11个居群的122个样品进行扩增,共得到150个扩增位点,其中多态性位点149个,多态位点百分率(PPL)为99.33%。POPGENE 分析显示,太行菊具有较高的遗传多样性(H=0.2149,I=0.3455)。沁阳市大西天居群的遗传多样性水平最高(H=0.1910,I=0.2969),山西陵川县大双村居群的遗传多样性水平最低(H=0.1356,I=0.2155)。Nei's遗传多样性分析表明,11个自然居群间出现了较高的遗传分化(基因分化系数Gst=0.2566,基因流Nm=1.4488)。生境的的片段化和基因流障碍可能是导致太行菊居群间遗传分化显著的主要原因。通过对太行菊居群遗传多样性和遗传结构的分析,该文提出了一些保护策略。

Abstract:

Using inter-simple sequence repeat(ISSR)analysis,the genetic diversity of 11 natural populations of Opisthopapus taihangensis were investigated. Ten ISSR primers were used to amplify 122 individuals of 11 natural populations. Using these primers,150 DNA fragments were produced,and 149 bands were polymorphic loci(PPL=99.33%). The result of POPGENE analysis indicated that genetic diversity of O. taihangensis was higher(I=0.2149,H=0.3455). Daxitian possessed the highest level of genetic diversity while Lingchuan population exhibited the lowest level of genetic diversity. A high level of genetic differentiation among 11 populations was detected based on Nei's genetic diversity analysis(Gst=0.2566,Nm=1.4488). The main factors responsible for the high degree of genetic differentiation among populations may result from habitat fragmentation and barriers of gene flow. Based on genetic information available for O. taihangensis,some conservation strategies proposed.