| 摘要: |
| 采用倍性分析和AFLP标记相结合,对滑皮金桔和变异单株金柑Ⅱ进行遗传鉴定分析。结果表明:滑皮金桔是2倍体,金柑Ⅱ是4倍体; 选用12对AFLP引物组合,就8份金柑资源进行遗传多样性分析及分类研究,共获得清晰可见的标记341,其中多态性标记95,占27.8%,供试材料相似系数在0.867～0.985之间,能将8个品种区分开,滑皮金桔与普通金柑相似性系数为96.8%,金柑Ⅱ与普通金柑相似性系数为95.8%,滑皮金桔与金柑Ⅱ相似性系数为95.7%。表明滑皮金桔和金柑Ⅱ是不同于普通金柑的变异新种质,可以直接作为品种资源加以利用。 |
| 关键词: 金柑 新种质 倍性分析 遗传鉴定 AFLP |
| DOI：10.3969/j.issn.1000-3142.2014.04.023 |
| 分类号:S666.9 |
| Fund project: |
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| Identification of Kumquats(Fortunella crassifolia)new varieties by ploidy analysis and AFLP |
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FU Cui-Na, XIAO Yuan-Hui, GAN Hai-Feng*, LEI Xin-Nan
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Guangxi Academy of Sepcialty Crops, Guilin 541004, China
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| Abstract: |
| To determine the genetic diversity of Kumquats new varieties. We employed ploidy analysis and 12 pairs of AFLP primer combinations on 8 Kumquats varieties. Ploidy analysis confirmed that Huapijingan was a diploid,Jingan Ⅱ was a tetraploid. AFLP results showed that: A total of 341 major AFLP bands were observed,and 95 band were polymorphism. Genetic similarities among all taxa ranged from 0.867 to 0.985. 8 varieties could be divided. Phenetic trees based on genetic similarities(UPGMA,N-J)were similar to known taxonomic relationships. the genetic similarity coefficient between Huapijingan and Jingan was 96.8%,the genetic similarity coefficient between Jingan Ⅱ and Jingan was 95.8%,the genetic similarity coefficient between Jingan Ⅱ and Huapijingan was 95.7%. Jingan Ⅱ and Huapijingan could be used as new cultivar. |
| Key words: Kumquats new varieties ploidy analysis genetic identification AFLP |
