梵净山冷杉和元宝山冷杉的叶绿体微卫星遗传多样性分析

韦 范<sup>1; 2</sup>; 张广荣<sup>1</sup>; 覃永贤<sup>1</sup>; 刘付永清<sup>1</sup>; 唐绍清<sup>1*</sup>

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梵净山冷杉和元宝山冷杉的叶绿体微卫星遗传多样性分析
韦范^{1, 2}, 张广荣¹, 覃永贤¹, 刘付永清¹, 唐绍清^1*
1. 广西师范大学生命科学学院, 珍稀濒危动植物生态与环境保护省部共建教育部重点实验室, 广西桂林 541004;2. 广西壮族自治区药用植物园, 南宁 530023

摘要:

梵净山冷杉和元宝山冷杉是极度濒危的国家一级重点保护植物。应用叶绿体微卫星标记(cpSSR)研究它们的遗传多样性,并与同属的广布种岷江冷杉的一个种群进行比较。结果表明:3对cpSSR引物(Pt63718、Pt30204和Pt71936)在这3种冷杉的249个个体中共检测到21个等位基因,组成35种单倍型; 它们的单倍型数和有效单倍型数分别为梵净山冷杉(No=12,Ne=3.92),元宝山冷杉(No=9,Ne=3.28),两者均低于广布种岷江冷杉(No=14,Ne=11.57); 梵净山冷杉和元宝山冷杉的稀有单倍型较少,其频率最高的单倍型分别出现在该种群的46.0%和44.1%的个体中; 梵净山冷杉(He=0.75)和元宝山冷杉(He=0.70)的单倍型多样性也低于岷江冷杉(He=0.97)。梵净山冷杉和元宝山冷杉的叶绿体微卫星遗传多样性水平低。

关键词: 梵净山冷杉元宝山冷杉叶绿体微卫星遗传多样性

DOI：10.3969/j.issn.1000-3142.2014.05.004

分类号:Q948.12; Q16

Fund project:

Genetic diversity at chloroplast microsatellites(cpSSR)in Abies fanjingshanensis and A. yuanbaoshanensis

WEI Fan^1,2, ZHANG Guang-Rong¹, QIN Yong-Xian¹, LIUFU Yong-Qing¹, TANG Shao-Qing^1*

1. Key Laboratory for Ecology of Rare and Endangered Species and Environmental Protection Co-established by Guangxi and the Ministry of Education, College of Life Sciences, Guangxi Normal University, Guilin 541004, China;2. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China

Abstract:

Abies fanjingshanensis and A. yuanbaoshanensis are critically endangered plants. We used chloroplast microsatellite marker to compare the genetic diversity of A. yuanbaoshanensis and A. fanjingshanensis with a widely distributed species A. fargesii var. faxoniana. A total of 21 alleles and 35 haplotypes was detected in 249 individuals from 3 pairs of primer(Pt63718,Pt30204 and Pt71936). The number of haplotypes(No),effective number of haplotypes(Ne)of A. fanjingshanensis(No=12,Ne=3.92)and A. yuanbaoshanensis(No=9,Ne=3.28)were lower than A. fargesii var. faxoniana. A. fanjingshanensis and A. yuanbaoshanensis had less rare haplotypes. The haplotype diversity(He)of A. fanjingshanensis(He=0.75)and A. yuanbaoshanensis(He=0.70)were also lower than A. fargesii var. faxoniana(He=0.97). A. fanjingshanensis and A. yuanbaoshanensis had a relatively low level of genetic diversity at chloroplast microsatellite.

Key words: Abies fanjingshanensis A. yuanbaoshanensis chloroplast microsatellite genetic diversity