| 摘要: |
| MADS-box基因参与植物多种发育过程,尤其在花发育过程中发挥着重要的调控作用。该研究以玉米不育系C48-2及保持系N48-2为材料,采用RT-PCR克隆mads3基因。序列分析发现,在不育系C48-2中该基因编码区发生单碱基突变,导致其推定的蛋白质中5个氨基酸序列发生改变。荧光定量PCR显示,相对于保持系,花粉母细胞时期和四分体时期mads3基因在C48-2下调表达,而单核期和双核期上调表达。这些结果为进一步了解玉米C型细胞质雄性不育的发生机制提供参考。 |
| 关键词: 玉米 CMS-C 花药 mads3基因 |
| DOI：10.3969/j.issn.1000-3142.2014.05.017 |
| 分类号:Q943.2 |
| Fund project: |
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| Cloning and expression analysis of mads3 in maize CMS-C sterile line and its maintainer line |
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WANG Ji-Yue, WANG Sheng-Qing, CAO Mo-Ju*
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Maize Research Institute of Sichuan Agricultural University/Key Laboratory of Crop Genetic Resource and Improvement,
Ministry of Education/Key Laboratory of Maize Biology and Genetic Breeding on Southwest,
Ministry of Agriculture, Chengdu 611130, China
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| Abstract: |
| MADS-box genes involve in various developmental processes in plant,and play an important role in the regulation of floral development. In this paper,mads3,a member of MADS box gene family was cloned by RT-PCR in maize. Sequence analysis showed that 8 sits single-base substitution mutations in CDS region were found in CMS-C line in comparison to maintainer line,which may result in 5 individual amino acides change. Furthermore,real-time qPCR analysis showed that mads3 was significantly down-regulated in CMS-C line at tetrad stage,and was significantly up-regulated at uninucleate stage. These results laid the foundation for further studying the relationship between MADS-box genes and CMS-C in maize. |
| Key words: maize CMS-C anther mads3 |
