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| 四川宜宾七种中药提取物体外抗菌活性研究 |
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李 莉1, 殷中琼1,2*, 贾仁勇1,2, 代如意1, 李 曼1, 蔡 红1
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1. 四川农业大学 动物医学院, 四川 雅安 625014;2. 四川农业大学 动物疫病与人类健康四川省重点实验室, 四川 雅安 625014
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| 摘要: |
| 用肉汤二倍稀释法和琼脂平板培养计数法,研究四川宜宾七种中药的提取物对大肠杆菌、沙门氏菌和巴氏杆菌的体外抑制作用。结果表明:黄柏提取物的抗菌活性最强,最小抑菌浓度(Minimum inhibitory concentration,MIC)为15.6～125 mg·mL-1,最小杀菌浓度(Minimum bactericidal concentration,MBC)为31.25～250 mg·mL-1; 栀子提取物抗菌活性次之,MIC为62.5～125 mg·mL-1,MBC为125～>250 mg·mL-1; 佛手提取物有较稳定的抗菌活性,MIC均为125 mg·mL-1,MBC为125 mg·mL-1和250 mg·mL-1; 姜黄、杜仲、何首乌、细毡毛忍冬的提取物抗菌活性相对较差,大部分MIC≥250 mg·mL-1。说明四川宜宾的黄柏和栀子的提取物抗菌活性较强,佛手提取物抗菌活性较稳定,有进一步研究的价值。 |
| 关键词: 四川宜宾 中药提取物 抗菌活性 MIC MBC |
| DOI：10.3969/j.issn.1000-3142.2014.05.024 |
| 分类号:Q946; S853.73 |
| Fund project: |
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| Antimicrobial activity in vitro of crude extracts from seven kinds of traditional Chinese medicine from Yibin in Sichuan |
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LI Li1, YIN Zhong-Qiong1,2*, JIA Ren-Yong1,2, DAI Ru-Yi 1, LI Man1, CAI Hong 1
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1. College of Veterinary Medicine Sichuan Agricultural University, Ya'an 625014, China;2. Key Laboratory of
Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China
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| Abstract: |
| The antimicrobial activities of crude extracts from seven kinds of traditional Chinese medicine from Yibin in Sichuan against E.coli,Salmonella and Pasteurella in vitro were investigated with serial two-fold broth dilution method and agar plate counting method. The results showed that the crude extract from Phellodendron amurense had the strongest antimicrobial activity,its Minimum inhibitory concentration(MIC)range was 15.6-125 mg·mL-1,its minimum bactericidal concentration(MBC)was 31.25-250 mg·mL-1. The antimicrobial activity of crude extract from Gardenia jasminoides was worse than Phellodendron amurense,its MIC was 62.5-250 mg·mL-1,and MBC was 125->250 mg·mL-1.The crude extract from Citrus medica var. sarcodactylis had stable antimicrobial activity,its MIC all was 125 mg·mL-1,MBC was 125 mg·mL-1 and 250 mg·mL-1. The other four extracts from Eucommia ulmoides,Fallopia multiflora、Curcuma longa,Lonicera similis were proved that their antimicrobial activities were not as strong as other three extracts. Most of their MIC were≥250 mg·mL-1. The results indicated that the extracts of Phellodendron amurense and Gadenia jasminoides had better bacteriostasis effect,and extract of Citrus medica var. Sarcodactylis was stable. They might be potential agents against bacterial disease,so they are worth of further study. |
| Key words: Yibin in Sichuan extracts from traditional Chinese medicine antimicrobial activity MIC MBC |
