24份甜樱桃材料自交不亲和S基因型鉴定

Identification of self-incompatibility genotypes of 24 sweet cherries

关键词:  甜樱桃  自交不亲和  S基因型

Key words:  sweet cherry  self-incompatibility  S-genotypes

摘要:

甜樱桃品种绝大部分自交不亲和,限制了甜樱桃的正确评价和合理利用,因此自交不亲和基因型的鉴定对于生产具有重要意义。以24个甜樱桃主栽品种为材料,用5对蔷薇科李属引物组合对24个甜樱桃品种进行了S等位基因的PCR扩增,克隆S基因的扩增片段,用核酸序列在GenBank上搜索,确定了5种S基因的核酸序列和大小。结果表明:PruC2+PruC4R引物组合扩增效果最好; 在琼脂糖凝胶上位置相同的扩增带其核酸序列相同,是同一种S基因; 5种S基因扩增片段的大小分别是S1为800 bp,S3为762 bp,S4为962 bp,S5为300 bp,S6为456 bp,S9为650 bp; 24个甜樱桃S基因型是红手球、早红宝石为S1S3,拉宾斯S1S4',红宝石S1S6,布鲁克斯S1S9,那翁S3S4,秦林、泰安大紫、先锋、早大果、丽珠、美早、5-106、左滕锦、桑提娜为S3S6,黑珍珠、红灯、萨米脱、秦樱为S3S9,胜利为S5S9,明珠、红蜜、雷尼、滨库为S6S9。

Abstract:

Sweet cherry exhibits the monofactorial gametophytic self-incocompatibility system, the correct evaluation and rational utilization on sweet cherry cultivars have been limited because of the self-incompatibility, therefore, determination of correct pollen incompatibility groups and assignment of cultivars to the groups are essential for good crop production. With 24 sweet cherry cultivars as materials, using 5 pairs of prunus primer combinations to amplify S-allele-specific PCR for 24 kinds of sweet cherries and S-genotypes of sweet cherry cultivars were identified, the S-gene amplified fragments were cloned and searched in GenBank, and combined with 5 primer combinations, S-genotype of 24 cultivars were identified. The conclusion was as follows: the same S genes displayed the same size fragments in electrophoresis; The sizes of S-gene amplification fragments were S1 800 bp, S3 762 bp, S4 962 bp, S5 300 bp, S6 456 bp,S9 650 bp. And the S genotypes of the tested self-incompatible cultivars were identified that Hongshouqiu and Early Ruby were S1S3; Lapins was S1S4; Ruby was S1S6; Brooks was S1S9; Napoleon was S3S4; Qinlin, Tartarian, Van, Zaodaguo, Lizhu, Meizao, 5-106 and Satonishiki the same as Santina were S3S6; Heizhenzhu, Hongdeng, Samituo and Qinying were S3S9; Victor was S5S9; Mingzhu, Hongmi and Rainier the same as Bing were S6S9.