稀土元素镧和铈对冬凌草再生植株生长及次生代谢产物的影响

Effects of rare-earth elements La3+and Ce3+ on the growth of Rabdosia rubescens regeneration plant and the accumulation of main secondary metabolites

关键词:  冬凌草  稀土元素  甲素  乙素  迷迭香酸

Key words:  Rabdosia rubescens  rare earth elements  oridonin  ponicidin  rosmarinic acid

摘要:

以冬凌草无菌苗为材料,在冬凌草再生植株时期,添加不同浓度的稀土元素镧和铈,用重量法测定其单株平均鲜重及干重,用高效液相法测定冬凌草甲素、乙素、迷迭香酸的含量,参考农作物、园艺作物种质评比的方法,根据冬凌草生产中各项指标的重要性,设置权重,计算加权后得到综合评分对其进行综合评价,探讨稀土元素镧、铈对冬凌草再生植株产量及次生代谢产物冬凌草甲素、乙素、迷迭香酸含量的影响。结果表明:空白对照组综合评分为62.99分,添加稀土元素铈的条件下,1 μmol·L^-1 CeCl₃·7H₂O评分最高为87.96分,添加稀土元素镧的条件下,5 μmol·L^-1 LaCl₃·6H₂O评分最高为74.44分,这表明CeCl₃·7H₂O对冬凌草再生植株生长及次生代谢产物积累的促进作用优于LaCl₃·6H₂O; 适宜浓度的镧、铈能促进冬凌草再生植株的生长及次生代谢产物冬凌草甲素、冬凌草乙素、迷迭香酸的合成,而高浓度10 μmol·L^-1 LaCl₃·6H₂O具有抑制的情况。该研究结果将对进一步研究稀土元素对冬凌草再生植株生长的促进作用机制奠定基础,为植物组织培养中如何科学合理地使用稀土元素提供理论依据。

Abstract:

Aseptic seedlings of Rabdosia rubescens were used as the experiment material. In R. rubescens regeneration period, the rare-earth elements La³⁺or Ce³⁺ with different concentrations were added in the culture medium. Fresh and dry weight of the plant were determined by gravimetric method. HPLC method was used for the determination of oridonin, ponicidin and rosmarinic acid content. We determined weight according to evaluating methods of agricultural and horticultural crops and the importance of indexes in the production of R. rubescens. The quality synthetic evaluation was also carried out according to weighted mark. The effects of rare earth elements La³⁺ and Ce³⁺ on R. rubescens tissue culture and the contents of oridonin, ponicidin, rosmarinic acid in R. rubescens were studied. The synthetic score of blank control group was 61.51 points,under the condition of 1 μmol·L^-1 CeCl₃·7H₂O, the score reached up to the highest value of 87.20 points. But under the condition of 5 μmol·L^-1 LaCl₃·6H₂O,the score 74.44 points. The results of this experiment showed that CeCl₃·7H₂O had more positive promoting effects on the growth of regeneration plant of R. rubescens and the accumulation of main secondary metabolites than LaCl₃·6H₂O. The optimal concentration of Ce³⁺or La³⁺ could promote the growth of regeneration plant of R. rubescens and secondary metabolites oridonin,ponicidin and rosmarinic synthesis. However,La³⁺ concentration in high level inhibited the growth of R. rubescens plant regeneration. The experiment would lay a foundation for further study on the promotion effect on the growth of regeneration plant of R. rubescens. The study of R. rubescens could provide theoretical basis for the rational utilization of rare earth elements.