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桂林毛村岩溶区典型植物叶片碳、氮、磷化学计量特征
杨 慧, 李青芳, 涂春艳, 曹建华
1. 广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室, 南宁 530005;2. 中国农业科学院甘蔗研究中心/ 农业部广西甘蔗生物技术与遗传改良重点实验室/广西农业科学院/ 广西甘蔗遗传改良重点实验室, 南宁 530007
摘要:
为探明甘蔗原种和地方种的遗传多样性和亲缘关系,以期筛选出优良甘蔗种质和优良杂交亲本。该研究对18份甘蔗原种和地方种的14个数量性状进行了表型遗传多样性分析。结果表明:通过14个数量性状的变异系数(coefficient of variance,CV)和性状之间的相关分析,18份甘蔗原种和地方种的数量性状遗传变异主要来自甘蔗蔗糖分、单茎重、叶宽、茎径和纤维分; 对14个数量性状进行主成分分析提取获得了4个主成分因子,分别命名为“品质因子”、“生长因子”、“成熟度因子”和“光合因子”,主成分因子累积贡献率达83.482%; 进一步通过对主成分因子开展综合评价分析,获得数量性状综合表型高于平均水平的10份材料,依次为Sampana→甜圪塔→合庆草甘蔗→桂林竹蔗→坦桑尼亚→芒戈→古芝蔗→大岛再来→托江红→春尼; 聚类分析基于不同的遗传距离可将18份种质聚为5个类别,潜在的优良杂交组合是Sampana和甜圪塔或Sampana和合庆草甘蔗,表明在甘蔗遗传育种亲本选择上既要考虑各性状主要因子的互补,又要保持一定的遗传距离。该研究认为,在甘蔗育种工作中,利用因子分析法进行表型遗传多样性分析,将更加有助于亲本和杂交组合的选择。
关键词:  甘蔗  数量性状  遗传多样性  因子分析
DOI:10.11931/guihaia.gxzw201403017
分类号:Q944, Q945.4
Fund project:
Phenotypic and genetic diversity of sugarcane ( Saccharum L. )protospecies and landraces
YANG Cui-Feng1, ZHANG Chun-Ya1, YANG Li-Tao1,2, LI Yang-Rui2*
1. Agricultural College/State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530005, China;2. Sugarcane Research Center, Chinese Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi), Ministry of Agriculture/Guangxi Academy of Agricultural Sciences/ Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China
Abstract:
In order to explore genetic diversity and relationship among different sugarcane species,select excellent sugarcane germplasm and parental combination for hybridization,the present study was conducted for genetic diversity analysis using 18 genotypes of protospecies and landraces of sugarcane(Saccharum spp.)with 14 quantitative traits. The results showed that the phenotypic and genetic diversity of sugarcane was highly significant in sucrose% cane,stalk weight,leaf width,stalk diameter and fiber% cane by coefficient of variation and correlation analysis of 14 quantitative traits. Four principal components of quantitative traits named quality factor,growth factor,maturity factor and photosynthetic factor,respectively,were extracted from principal component analysis, and the cumulative contribution rate of four principal components was 83.48%. Ten desirable genotypes of sugarcane had higher values of quantitative traits than the averages in factor analysis in turn as follows:Sampana→Tiangeda→Heqingcao Ganzhe→Guilin Zhuzhe→Tanzania→Mango→Guzhizhe→Dadaozailai→Tuojianghong→Chunnee. The 18 accessions could be divided into five clusters based on the genetic distance,and potential hybrid may be developed by crossing Sampana with Tiangeda and/or Sampana with Heqingcao Ganzhe. It suggested that we should not only consider the complementary of main traits,but also genetic distance when we select parents for crossing in sugarcane breeding. The present study proved that factor analysis was good to help the parental selection and determine crossing combinations in sugarcane breeding program.
Key words:  sugarcane  quantitative traits  genetic diversity  factor analysis
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