灵武长枣果实质膜和液泡膜H+-ATPase和H+-PPase活性与果实糖分积累

H+-ATPase and H+-PPase activities of plasmalemma and tonoplast and sugar accumulation in fruit of Ziziphus jujuba cv. Lingwuchangzao

关键词:  灵武长枣  质膜  液泡膜  H⁺-ATPase  H⁺-PPase  糖积累

Key words:  Ziziphus jujuba cv. Lingwuchangzao  plasmalemma  tonoplast  H⁺-ATPase  H⁺-PPase  sugar accumulation

摘要:

以不同发育时期灵武长枣(Ziziphus jujuba cv. Lingwuchangzao)的果实为材料,通过测定与分析果肉组织中细胞质膜、液泡膜H⁺-ATPase和H⁺-PPase活性、果实糖分含量变化,研究了灵武长枣果实质膜、液泡膜H⁺-ATPase和H⁺-PPase活性与糖积累特性的关系。结果表明:(1)果实第二次快速生长期之前主要积累葡萄糖和果糖,之后果实迅速积累蔗糖,葡萄糖和果糖含量则逐渐下降,成熟期果实主要积累蔗糖。(2)在果实发育的缓慢生长期S1,质膜H⁺-ATPase活性最低; 第一次快速生长期,质膜H⁺-ATPase活性最高; 缓慢生长期S2,其活性降低; 第二次快速生长期,质膜H⁺-ATPase活性升至次高; 完熟期,质膜H⁺-ATPase活性下降幅度较大。(3)在果实发育过程中,液泡膜H⁺-ATPase和H⁺-PPase活性的变化趋势相似。缓慢生长期S1,液泡膜H⁺-ATPase和H⁺-PPase活性较低; 从缓慢生长期S1至第一次快速生长期缓慢下降至最低; 从第一次快速生长期开始,液泡膜H⁺-ATPase和H⁺-PPase活性呈现为逐渐增高的变化趋势; 除第二次快速生长期以外,液泡膜H⁺-PPase活性始终高于H⁺-ATPase。由此推测,质膜H⁺-ATPase和液泡膜H⁺-ATPase、H⁺-PPase对灵武长枣果实糖分的跨膜次级转运起到重要的调控作用。

Abstract:

In order to study the relation between the H⁺-ATPase and H⁺-PPase activities of plasmalemma and tonoplast and the sugar accumulation in fruit of Ziziphus jujuba cv. Lingwuchangzao, the H⁺-ATPase and H⁺-PPase activities of plasmalemma and tonoplast and the sugar content in fruit were determined with different developmental periods fruit as materials. The results were as follows:(1)The fruit mainly accumulated glucose and fructose before the second rapid growth period, sucrose accumulated rapidly after the second rapid growth period, at the same time, the content of glucose and fructose decreased gradually,the mature period fruit mainly accumulated sucrose.(2)The plasmalemma H⁺-ATPase activity was the lowest in the slower growth period S1 and the highest in the first rapid growth period,but it droped in the slower growth period S2 and rose to higher in the second rapid growth period and droped substantially in the mature period.(3)The variation trend of tonoplast H⁺-ATPase activity was similar to tonoplast H⁺-PPase activity during fruit development. Tonoplast H⁺-ATPase and H⁺-PPase activities were lower in the slower growth period S1,they droped slowly to the lowest from the slower growth period S1 to the first rapid growth period and increased gradually from the first rapid growth period,tonoplast H⁺-PPase activity was higher throughout than tonoplast H⁺-ATPase activity except the second rapid growth period. Hence, the plasmalemma H⁺-ATPase and the tonoplast H⁺-ATPase and H⁺-PPase played an important regulative role in secondary active transport sugar across membrane in fruit of Z. jujuba cv. Lingwuchangzao.