| 摘要: |
| 以陕南地区的3种主栽柑橘离体叶片为材料,分别以5、0、-5和-10 ℃进行低温处理,分析与耐寒性相关的生理响应,并采用隶属函数法分析3种柑橘的抗寒能力以及通过主成分分析评价各个测定指标的贡献率。结果表明:随着处理温度的降低,3种柑橘离体叶片均依次出现叶片卷曲、叶片细胞内的水分外渗,细胞内结冰导致叶片僵硬和最终的叶片内外结冰情况,叶片形态变化结果显示3种柑橘抗寒能力强弱依次为日南1号>兴津>泛亚特早。随处理温度的降低,3种柑橘离体叶片的相对电导率(REC)和丙二醛(MDA)均从0 ℃处理时呈显著升高趋势,-10 ℃处理时达到最高值,同一低温处理条件下REC和MDA值的大小顺序均为泛亚特早>兴津>日南1号。O-2·、H2O2和可溶性糖含量总体保持升高的趋势,而脯氨酸含量呈先显著升高后降低的趋势。抗氧化酶SOD、CAT和APX的活性及抗氧化物质ASC含量均呈现先升高后下降的趋势。REC和MDA综合指标的累积贡献率高于85%,具有较强的代表性,可作为柑橘抗寒性分析的重要指标,平均隶属度分析结果表明3种柑橘抗寒能力依次为日南1号>兴津>泛亚特早。 |
| 关键词: 柑橘, 低温, 相对电导率, 隶属函数, 脯氨酸 |
| DOI：10.11931/guihaia.gxzw201507029 |
| 分类号:Q945.78 |
| 文章编号:1000-3142(2016)02-0208-08 |
| Fund project:陕西省自然科学基金(2014JQ3113); 陕西省教育厅自然科学基金(14JK1158); 陕西理工学院科研基金(SLGKY15-41)[Supported by the Natural Science Foundation Research Project of Shaanxi Province(2014JQ3113); Natural Science Research Project in Shaanxi Province Department of Education(14JK1158); Scientific Research Fund from Shaanxi University of Technology(SLGKY15-41)]。 |
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| Low temperature stress affected physiological characters in three varieties of Citrus leaves |
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JIANG Jing-Long1, LI Li2, ZHAO Hua1, LI Xin-Sheng3, SHEN Ji-Xue1, XU Wei-Ping1
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1. School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China;2. Chemical and Environmental Sciences, Shaanxi University of Technology, Hanzhong 723001,
China;3. Shaanxi Key Laboratory of Bio-resources, Hanzhong 723001, China
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| Abstract: |
| Three cultivars of Citrus planted in the southern area of Shaanxi Province were used as experimental materials, and their detached leaves were treated at 5 ℃, 0 ℃, -5 ℃ and -10 ℃to analyze the physiological responses related with cold hardiness of Citrus under low temperature stress. Meanwhile the method of subordinate function was used to compare the cold resistance abilities of Citrus and measure parameter and their contribution rate were analyzed by using principal components analysis. The results showed that three varieties of Citrus leaves in vitro were successively appear leaf curl, water leakage inside the leaf cells, intracellular freezing and eventually lead to stiff blades and external icing with the processing temperature decreases. Changes results of leaf morphology showed that the order of cold tolerance in three varieties of Citrus was Nichinan 1>Okitsu>Fanyatezao. The relative electric conductivity(REC)and malondialdehyde(MDA)of detached leaf all increased obviously under the temperature of 0 ℃ and reached the max value upon the temperature of -10 ℃ in three varieties of Citrus as temperature decreased. REC and MDA value at the same low temperature conditions was the order of Fanyatezao>Okitsu>Nichinan 1. The content of O-2·, H2O2 and soluble sugar all kept the overall rising trend and proline significantly increased after the first decreased. The activities of SOD, CAT and APX as well as the content of ASC generally increased first and then decreased. The results showed that the cumulative contribution of REC and MDA composite indicator was higher than 85% and had strong representation as citrus cold analysis of key indicators. The results identified by the average degree of membership hardiness indicated that the order of cold tolerance in three varieties of Citrus was Nichinan 1>Okitsu>Fanyatezao. |
| Key words: Citrus, low temperature, relative electric conductivity, subordinate function, proline |
