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| 不同铅水平下紫茎泽兰细胞内铅的分布和化学形态的分析 |
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刘小文1,2,3, 齐成媚1, 李 园2,3, 颜冬冬2,3, 王秋霞2,3, 郭美霞2,3, 曹坳程2,3*
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1. 湖南科技学院 化学与生物工程学院, 湖南 永州 425199;2. 植物病虫害生物学国家重点实验室,
北京 100193;3. 中国农业科学院植物保护研究所, 北京 100193
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| 摘要: |
| 该研究运用差速离心法和化学试剂逐步提取法,分析了重金属铅在紫茎泽兰亚细胞内的分布和主要化学形态。结果表明:随着Pb浓度的升高,紫茎泽兰的叶、根、茎中各亚细胞组分Pb含量逐渐增加; 紫茎泽兰中的Pb在叶片分布于可溶性部分和细胞壁中,两者占总量的75.34%～84.63%; 茎也主要分布于可溶性部分和细胞壁中,占总量的36.10%～57.14%和20.07%～36.52%; 而在根中则富集于细胞壁和可溶性部分,分别占39.2%～49.78%和28.27%～37.62%,其他细胞器中的Pb含量均较少。紫茎泽兰叶中的Pb以盐酸提取态和水提取态为主,两者占总量的58.74%～73.04%; 茎中的Pb以醋酸提取态和氯化钠提取态为主; 而根中的Pb则以醋酸提取态和盐酸提取态占优势,两者占总量的39.15%～52.91%。 |
| 关键词: 铅, 紫茎泽兰, 亚细胞分布, 化学形态 |
| DOI：10.11931/guihaia.gxzw201404010 |
| 分类号:Q945.79 |
| 文章编号:1000-3142(2016)03-0335-07 |
| Fund project:国家公益性行业(农业)科技专项(201103027); 湖南省教育优秀青年项目(14B071); 中国博士后科学基金第56批面上项目(2014M561110); 湖南省自然科学基金(13JJ6080); 湖南省重点学科建设项目(2011-76); 湖南省高校科技创新团队支持计划项目(2012-318)[Supported by National Public Welfare Fund for Agricultural Research(201103027); Preject for Advanced Youth of Hunan Provincial Education Department(14B071); Postdoctoral Science Foundation of China(2014M561110); Key Discipline Construction Project of Hunan Province(2011-76); Science and Technology Innovation Team Plan of Hunan Provincial Colleges and Universities(2012-318)]。 |
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| Subcellular distribution and chemical forms of lead in Eupatorium adenophorum at different lead levels |
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LIU Xiao-Wen1,2,3, QI Cheng-Mei1, LI Yuan2,3, YAN Dong-Dong2,3,
WANG Qiu-Xia2,3, GUO Mei-Xia2,3, CAO Ao-Cheng2,3*
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1. College of Biological and Chemical Engineering, Hunan University of Science and Technology, Yongzhou 425199, China;2. State Key Laboratory for Biology of Diseases and Insert Pests, Beijing 100193, China;3. Institute of Plant
Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
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| Abstract: |
| The differential centrifugation technique and sequential chemical extraction method were used to study the subcellular distribution and chemical forms of lead in the roots, leaves and stems of Eupatorium adenophorum. The results showed that with the increasing of lead levels, the contents of lead in subcellular distribution of leaves, stems and roots significant increased. The lead distributed mainly in the soluble partition and cell wall in leaves, occupied 75.34%-84.63% of the total. Lead distributed in stems were similar to it in leaves. It mainly accumulated in the soluble partition and cell wall, which occupied 36.10%-57.14% and 20.07%-36.52%, respectively. And it distributed mainly in cell wall and soluble partition in roots, which occupied 39.20%-49.78% and 28.27%-37.62%, respectively. There was less lead in other organelles. The chemical forms of lead in leaves of E. adenophorum were mainly in HCl and water, occupied 58.74%-73.04% of the total. In stems, the main chemical forms were HAc and NaCl, and the chemical forms of HAc and HCl were predominant in roots, occupied 39.15%-52.91% of the total. |
| Key words: lead, Eupatorium adenophorum, subcellular distribution, chemical forms |
