不同干扰生境下河口莲座蕨遗传多样性分析

Analysis on the genetic diversity of Angiopteris hokouensis in habitats under different disturbances

关键词:  河口莲座蕨, 干扰, ISSR, 遗传多样性

Key words:  Angiopteris hokouensis, disturbance,ISSR, genetic diversity

摘要:

该研究利用 ISSR 分子标记,对不同生境下的6个河口莲座蕨种群进行遗传多样性分析。结果表明:从44条ISSR引物中筛选出8条能够扩增出清晰、稳定条带的引物,对6个河口莲座蕨种群进行基因组 DNA 扩增,共扩增出144条带,其中多态性条带119个,多态性条带比率为93.7%,Nei's遗传多样性指数(H)为0.296,Shannon多样性指数(I)为0.457,遗传分化指数(Gst)为0.152 0,种群遗传一致度和遗传距离分别为0.913 8～0.954 8和0.058 4～0.090 1; UPGMA聚类分析表明,种群间遗传距离与空间距离和生境类型有关。河口莲座蕨在不同干扰程度的生境中,种群具有高水平的遗传多样性,一定强度或频率的干扰生境中,种群遗传多样性较高,与海拔、坡度和坡向无相关性。

Abstract:

Genetic diversity of 6 Angiopteris hokouensis populations collected from different habitats were analyzed by means of intersimple sequence repeat(ISSR)markers, and 8 proper primers with rich polymorphism and stable bands were selected from a total of 44 ISSR's primers. The genome DNAs of the 6 populations were amplified to a total of 144 putative bands by the 8 primers, in which 96 were polymorphism bands. Polymorphism rate was 93.7%; Nei's gene diversity index was 0.296, Shannon information index was 0.457, and Gst was 0.152 0. The genetic distance coefficient and the genetic similarity were 0.058 4-0.090 1 and 0.913 8-0.954 8. Cluster analysis by UPGMA indicated that the genetic distance between the populations related to spatial distance, as well as to habitats. The results showed that A. hokouensis populations had high levels of genetic diversity at disturbed habitats. Even there was no significant differentiation of genetic diversity among populations,the populations in moderate disturbed habitats had higher genetic diversity. The genetic diversity was not significantly related toaltitude,slope and aspect.