| 摘要: |
| 锐药竹(Oxytenanthera abyssinica)是非洲竹区最重要的竹种,对当地民众生活、生产和环保具有重要作用,也是保护当地环境的重要组成成分。然而,对这样重要的竹种,非洲内、外均少见其遗传研究报道。该研究对采自埃塞俄比亚的同一居群锐药竹少见的开花结实种子,采用“林木种子检验规程”国家标准,测定种子的主要性状,并对其遗传品质进行了分析。结果表明:锐药竹的种子长度为18.2 mm,宽度为3.3 mm,千粒重为117.5 g,室内发芽率为66.5%。性状变异与其他竹类相近,变异不大。将这批种子育苗15 000多株,随机选择39株,典型选择25个叶片大的植株,共64个单株,采集叶样,运用扩增片段长度多态性(AFLP)分子标记技术进行遗传多样性检测。筛选E-ACA/M-CAA等8对引物对样品经行扩增,电泳分离,利用GeneScan 3.1软件共统计到1 728条谱带,计算出其多态位点百分率90.28%,Nei's基因多样性指数0.246 3,Shannon多样性指数0.362 3,为遗传多样性大的类型; 采用单匹配相似系数法对样品谱带进行UPGMA聚类,取SM=0.76,可将分析样品聚为3类,结合表型进一步进行种质挖掘研究。该研究结果为其生物多样性保育及选育优良品种提供了科学依据。 |
| 关键词: 非洲竹区, 种子性状, 种子批, AFLP, 种质资源 |
| DOI：10.11931/guihaia.gxzw201411038 |
| 分类号:Q943.1 |
| 文章编号:1000-3142(2016)08-0943-06 |
| Fund project:国家“948”项目(非洲重要特有经济竹子种质资源引进)(2012-4-48)[Supported by the National Program of Introducing Advanced Agricultural Science and Technology from Africa(2012-4-48)]。 |
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| Seed characters and genetic variation of Oxytenanthera abyssinica |
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LIAN Chao1, ZHANG Guo-Wu2, FENG Yun1, RAN Hong1,
ZHANG Ying1, GUO Qi-Rong1*
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1. International Center for Bamboo and Rattan, SFA Key Laboratory of Bamboo and Rattan Science and Technology, Beijing
100102, China;2. China Eucalyptus Research Centre, State Forestry Administration, Zhanjiang 524022, China
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| Abstract: |
| Oxytenanthera abyssinica is the most important indigenous bamboo in Africa. It plays an important role in local life, production and environment protection. However, the study of its genetic variation is little. We studied seed characters and its variation, as well as the genetic variation of its seedlings abide by GB2772-1999. The length of seeds was 18.2 mm, width was 3.3 mm, 1 000 seed mass was 117.5 g and germination percentage was 66.5%. The results showed that little variation occurred in its seed characters, and variation degree was similar to that of other bamboos. More than 15 000 seedlings was cultured, leaves of 64 individuals were chosen as test material typically, 39 of them were chosen randomly and 25 individuals with big leaves. AFLP was applied to test its genetic variation, eight pair of primers are used. After electrophoretic separation, 1 728 lines was calculated by GeneScan 3.1. It occurred that its PPB was 90.28%, Nei's gene diversity index was 0.243 6, Shannon index was 0.362 3. The result showed that the genetic variations was abundant in O. abyssinica. Based on simple matching coefficient(SM), the individuals were clustered by UPGMA cluster analysis. It indicated that the SM index ranged from 0.73 to 0.85. The samples can be clustered into three classes when SM similarity coefficient is 0.76. Combine phenotypic and physiological indicators systematic germplasm exaviation is further needed. This study will provide scientific information for biodiversity breeding and improved breeds. |
| Key words: African bamboo area, seed characters, seed lot, AFLP, germplasm resource |
