响应面法优化干花豆总黄酮提取工艺研究

Optimization of extracting total flavonoids from Fordia cauliflora by response surface methodology

关键词:  干花豆, 总黄酮, 响应面法, 抗氧化性

Key words:  Fordia cauliflora, flavone, response surface methodology, antioxidant activity

摘要:

干花豆(Fordia cauliflora)的主要有效成分为黄酮类、生物碱、有机酸等,具有益智、抗衰老、抗炎等作用。目前的研究主要集中在化学成分及药理活性等方面,对总黄酮成分提取工艺优化报道较少。该研究以新鲜干花豆为材料,以总黄酮提取量为评价指标,在提取温度、料液比、乙醇浓度和提取时间单因素实验基础上,采用响应面法优化了干花豆总黄酮提取工艺,同时测定了总黄酮对1, 1-二苯基苦基苯肼自由基(DPPH·)和羟基自由基(·OH)清除能力。结果表明:干花豆总黄酮提取最佳工艺条件为提取温度78 ℃、料液比为1:30(g·mL^-1)、乙醇浓度71%和提取时间为187 min。在此条件下,总黄酮得率预测值为10.61 mg·g^-1,实际为10.53 mg·g^-1,理论值与预测值的相对误差为0.76%; 干花豆总黄酮对DPPH和OH自由基清除能力IC₅₀值分别为14.09和78.43 μg·mL^-1,弱于Vc(8.11和67.95 μg·mL^-1)。该提取工艺稳定合理,准确可靠,是提取干花豆总黄酮的可行方法。该研究结果为干花豆中总黄酮成分的进一步开发利用奠定了基础。

Abstract:

Fordia cauliflora is the genus Fordia in the family Leguminosae, which contains flavonoids, alkaloids, organic acids and so on, possesses various activities such as beneficial wisdom, anti-aging, anti-inflammatory. The current research of F. cauliflora mainly focuses on the study of chemical constituents and pharmacological activities, there is little report about the optimization of extraction process of flavonoids. In order to determine the best extraction technology of total flavonoids from F. cauliflora and evaluate their antioxidant activity, the extraction temperature, solid-liquid ratio, ethanol concentration and extraction time as single factor were tested and then the extraction process of the total flavonoids from F. cauliflora was optimized by response surface designs based on single factor experiments. In addition, the antioxidant activity of flavonoids from F. cauliflora was measured by using 1,1-diphenyl-2-picryhydrazyl radical(DPPH·)and hydroxyl radical(·OH)scavenging. The results showed that total flavonoids of optimum extraction conditions of F. cauliflora were as follows: extraction temperature 78 ℃, solid-to-liquid ratio of 1:30(g·mL^-1), the alcohol concentration for 71%, extraction time 187 min. Under these conditions, an extraction rate of 10.53 mg·g^-1 for total flavonoids from F. cauliflora was obtained, compared with the predictive value of 10.61 mg·g^-1, the relative error was 0.76%. The IC₅₀ values of scavenging DPPH and OH radical were 14.09 and 78.43μg·mL^-1, weaker than that of Vc(8.11 and 67.95 μg·mL^-1). The extraction process was stable and reasonable, accurate and reliable, and it can be used to extract the total flavonoids from F. cauliflora. The results will provide basis for the further development and utilization of flavonoids of F. cauliflora.