摘要: |
水分亏缺是限制马缨杜鹃园林开发应用的关键因子,bHLH转录因子在植物生长发育和胁迫响应过程中发挥重要调控作用。以马缨杜鹃基因组文件和干旱胁迫处理转录表达数据为材料,运用生物信息学方法鉴定并分析了马缨杜鹃bHLH家族的成员、基因结构、保守结构域、系统发育、蛋白理化性质、亚细胞定位、顺式作用元件、蛋白互作网络及表达模式等特征。结果表明:共鉴定出116个马缨杜鹃bHLH家族基因,通过系统发育分析将其划分为17个亚家族;各亚家族成员相对保守,绝大多数bHLH基因同时含有Motif1,2两个共同组成bHLH保守结构域的保守基序;马缨杜鹃bHLH家族成员属于弱酸性亲水蛋白,基因主要在细胞核内行使功能;启动子区域含丰富的与植物生长发育(4.58%)、激素响应(33.54%)、光响应(43.39%)和胁迫响应(18.49)相关的元件;光合调节、活性氧清除、类黄酮等次生代谢物生物合成和气孔发育等系统等多种生理过程和代谢途径参与了干旱胁迫响应;马缨杜鹃bHLH转录因子DUH003369、DUH020758和DUH015020可能在植物抗干旱胁迫过程中发挥重要调节作用。研究结果为进一步研究马缨杜鹃bHLHs的生物学功能提供了信息参照,也为培育马缨杜鹃优良园艺品种提供了靶向基因资源。 |
关键词: 马缨杜鹃,bHLH,生物信息学,干旱胁迫,表达模式 |
DOI:10.11931/guihaia.gxzw202303024 |
分类号: |
Fund project:国家自然科学基金(32260097) |
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Identification and expression analysis of bHLH transcription factors family in Rhododendron delavayi |
WANG Hongfei1, OU Jing1*, WANG Xiaojing2, KE Ke3, FAN Yu1, ZHOU Yumei1, FAN Xinyue1
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1.College of Forestry,Guizhou University,Guiyang ,China;2.College of Tea,Guizhou University,Guiyang ,China;3.College of Agricultural,Guizhou University,Guiyang ,China
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Abstract: |
Water deficit is a key factor limiting the development and application of Rhododendron delavayi and other alpine rhododendron gardens. bHLH transcription factor plays an important role in plant growth and stress response. In this study, based on the genome files of Rhododendron delavayi and the transcription and expression data under drought stress, the members, gene structure, conserved domains, phylogeny, protein physicochemical properties, subcellular localization, cis-acting elements, protein interaction network and expression pattern of bHLH family genes in Rhododendron delavayi were identified and analyzed by bioinformatics methods. the result showed that 116 bHLH gene were identified and 17 subfamilies were identified by phylogenetic analysis; most bHLH genes contain two motifs, Motif1 and Motif 2, which together constitute the conserved domain of bHLH; the bHLH family members of Rhododendron delavayi belong to weakly acidic hydrophilic proteins; the gene mainly functions in the nucleus; the promoter region contains abundant elements related to plant growth and development (4.58%), hormone response (33.54%), light response (43.39%) and stress response (18.49); many physiological processes and metabolic pathways, such as photosynthetic regulation, active oxygen scavenging, biosynthesis of secondary metabolites such as flavonoids and stomatal development, are involved in drought stress response; the bHLH transcription factors DUH030431, DUH030504 and DUH003369 may play an important role in plant drought resistance. These results provide information reference for further study of biological function of Rhododendron delavayi bHLHs, and also provide targeted gene resources for breeding excellent horticultural varieties of Rhododendron delavayi. |
Key words: Rhododendron delavayi, bHLH, bioinformatics, water deficiency, expression patterns |