| 摘要: |
| 植物染色体的倍性维持和变化受环境因素影响,组培再生过程由于培养条件等因素往往导致染色体的结构和倍性变化。为探索组培条件下山茶种质的倍性变化,该研究利用山茶种质的愈伤组织诱导体系,通过流式细胞仪分析倍性变化情况,并结合秋水仙素处理对组培再生条件下倍性的稳定性和变化进行分析。结果表明:(1)10个山茶种质中6个为二倍体,2个为四倍体,1个为六倍体和1个为十倍体,在组培诱导愈伤及再生过程中不同倍性的种质材料能够保持稳定的倍性。(2)获得了秋水仙素处理的最适诱导条件,即培养基配方为秋水仙素浓度20 mg·L-1,愈伤增殖培养10 d。(3)对56个独立组织样品(含愈伤和芽)开展了倍性检测发现,有38个组织样品的倍性在1.5～2.5倍之间,11个组织样品产生了低于1.5倍性的特异现象。该研究结果进一步探索了不同山茶种质之间的倍性关系,为山茶属植物的倍性调控和多倍体诱导提供了理论基础。 |
| 关键词: 山茶属, 染色体倍性, 组织培养, 秋水仙素, 流式细胞术 |
| DOI：10.11931/guihaia.gxzw202305077 |
| 分类号:Q943 |
| 文章编号:1000-3142(2024)07-1299-08 |
| Fund project:国家自然科学基金(32271839)。 |
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| Maintenance and variation of chromosome ploidy under tissue culture conditions of different Camellia germplasms |
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LI Yaxuan1,2, LI Xinlei1, LI Jiyuan1, YIN Hengfu1*
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1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese
Academy of Forestry, Hangzhou 311400;2. Nanjing Forestry University, Nanjing 210037
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| Abstract: |
| The maintenance and variation of ploidy in plants are influenced by environmental factor, and the tissue culture regeneration can often cause changes of chromosome structures and ploidy levels. In order to explore the ploidy variation of Camellia germplasm under tissue culture conditions, the callus induction system of Camellia germplasm was used to analyze the ploidy changes by flow cytometry, and the stability and variation of the ploidy under tissue culture generation were analyzed in combination with colchicine treatment. The results were as follows:(1)Six of ten of Camellia germplasms were diploid, two of them were tetraploid, one hexaploid and one decaploid, and the germplasm materials largely maintained stable ploidy levels during the tissue regeneration processes.(2)The optimum conditions for colchicine treatment were obtained, that is, the callus was cultured in treatment medium containing colchicine at 20 mg·L-1 for 10 d, followed by regeneration in tissue-differentiation culture.(3)The ploidy analyses were carried out on the treated tissues in different differentiation states, and the results showed that most of the independent tissues(including 56 callus and shoots)were found to be aneuploids, 38 tissues had ploidy between 1.5 to 2.5, and 11 tissues produced less than 1.5. This study further explores the ploidy relationship between different Camellia germplasms, and provides a reference for an in-depth study of ploidy regulation and polyploid induction of Camellia. |
| Key words: Camellia, chromosome ploidy, tissue culture, colchicine, flow cytometry |
