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| 水稻OsACS2基因结构和功能的生物信息学分析 |
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杨欣月1,和月英1,杨傲1,江丽慧1,孙晓茜1,朱振豪1, 万媛媛2,郭力维1,彭晟1,杜云龙1*
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1.云南农业大学 植物保护学院, 昆明 650201；2.云南农业大学 经济管理学院, 昆明 650201
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| 摘要: |
| 为了研究水稻OsACS2基因的结构特征及其在水稻发育中的功能，该研究通过生物信息学方法对OsACS2及其在水稻、拟南芥、玉米、小麦、大麦、番茄和马铃薯中同源蛋白的理化性质、结构和亲缘关系进行分析，对水稻植株用植物激素脱落酸(ABA)及茉莉酸甲酯(MeJA)进行处理，利用real-time PCR分析OsACS2基因在水稻根与叶中的表达水平。结果表明：（1）OsACS2基因在水稻、拟南芥、小麦、番茄、马铃薯、玉米以及大麦中有34个同源基因；（2）OsACS2与玉米、小麦中的同源蛋白具有更近的亲缘关系；（3）OsACS2蛋白及其同源蛋白具有相似的亚细胞定位、含有一个保守的AMP (adenosine monophosphate)结合结构域，具有相似的二级结构及三级结构；（4）OsACS2基因的启动子中有ABA及MeJA响应元件，ABA的处理增加了OsACS2在根中的表达，降低了OsACS2在叶中的表达水平，而MeJA的处理使OsACS2的表达水平在根中降低，但增加了叶中的表达水平。该研究为深入解析OsACS2及同源基因的生物学功能建立理论基础。 |
| 关键词: OsACS2 线粒体 AMP结合结构域 水稻 茉莉酸 脱落酸 |
| DOI：10.11931/guihaia.gxzw202503013 |
| 分类号: |
| Fund project:国家自然科学基金(32260085，32460083)；中央引导地方科技发展资金自由探索类基础研究项目(202407AA110006)；国家重点研发计划(2023YFD1400204)；云南省应用基础研究计划重点项目(202301AS070082)；云南省中青年学术和技术带头人后备人才计划(202205AC160076)。 |
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| Structural characteristics and functional analysis of rice OsACS2 and its homologous genes |
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YANG Xinyue1, HE Yueying1, YANG Ao1, JIANG Lihui1, SUN Xiaoqian1, ZHU Zhenhao1, WAN Yuanyuan2, GUO Liwei1, PENG Sheng1, DU Yunlong1*
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1.College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China ； 2.College of Economics and Management, Yunnan Agricultural University, Kunming 650201, China
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| Abstract: |
| To study the structural characteristics and functions of rice OsACS2 gene in rice development, the physicochemical properties, structure, and phylogenetic relationships of OsACS2 and its homologous proteins in rice, Arabidopsis, maize, wheat, barley, tomato and potato were analyzed using bioinformatics methods. Rice plants were treated with phytohormones abscisic acid (ABA) and methyl jasmonate (MeJA), and the expression levels of OsACS2 gene in rice roots and leaves were analyzed using real-time PCR. The results were as follows: (1) There are 34 OsACS2 homologous genes were found in rice, Arabidopsis thaliana, wheat, tomato, potato, maize, and barley; (2) OsACS2 shows a closer relationship with its homologs in maize and wheat; (3) The OsACS2 protein and its homologs show similar subcellular localization, contain a conserved AMP (adenosine monophosphate)-binding domain, and have similar secondary and tertiary structures; (4) The promoter of the OsACS2 gene contains ABA and MeJA responsive elements, ABA treatment increased the expression of OsACS2 in roots and decreased its expression level in leaves, MeJA treatment resulted in a decrease in the expression level of OsACS2 in roots, but an increased level in leaves. This study establishes a theoretical basis for further understanding the biological functions of OsACS2 and homologous genes. |
| Key words: OsACS2 mitochondrion AMP-binding domain Rice jasmonic acid abscisic acid |
