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| 基于改良原位培养的红树林可培养细菌多样性及抑菌活性研究 |
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李王靖1,2,3, 李蜜1,2,3, 高梦蝶1,2,3, 徐淑芬1,2,3, 刘永宏1,2,3, 高程海1,2,3, 易湘茜1,2,3
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(1. 广西中医药大学 海洋药物研究院/药学院,南宁 530200；2. 广西海洋药物重点实验室/海洋中药资源高效利用广西高校工程研究中心,南宁 530200；3. 北部湾海洋药物和生物制品开发利用工程技术创新中心,南宁 530200）
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| 摘要: |
| 为探究具有抑制人类病原菌活性的海洋细菌资源，该研究采用改良原位培养装置，从北部湾10种红树根际土壤中富集海洋可培养细菌，通过16S rRNA基因序列鉴定可培养细菌种属，并采用滤纸片扩散法筛选具有抑制人类病原菌活性的海洋可培养细菌。结果表明：（1）获得240种海洋可培养细菌，隶属于4门8纲27目54科95属，链霉菌属为优势属，占26.7%。（2）16S rRNA相似度结果显示，13株海洋可培养细菌可能为新物种，其中菌株GXIMD 04217与标准菌株Photobacterium lucens CAIM 1938T的16S rRNA最高相似度为92.94%。（3）25种海洋可培养放线菌对至少1种人类致病菌有显著抑制活性，其中GXIMD 04328和GXIMD 04558对耐甲氧西林金黄色葡萄球菌、马尔尼菲蓝状菌的抑菌圈分别达（23.5±2.5） mm和（21.2±0.4） mm。（4）7种海洋可培养细菌具广谱抑制活性，可抑制多种人类致病菌。综上可见，采用改良培养装置从红树根际土壤中获得多样性丰富的可培养细菌，是发现海洋新型菌株和活性菌株的有效途径。 |
| 关键词: 红树土壤，原位培养装置，海洋可培养细菌，抑制人类病原菌，放线菌 |
| DOI：10.11931/guihaia.gxzw202507044 |
| 分类号: |
| Fund project:广西重点研发计划项目（AB24010109）；国家自然科学基金（42466005）；广西青年岐黄学者培养项目（GXQH202412）；广西中医药大学岐黄工程高层次人才团队培育项目（202404）；广西中医药大学高层次人才培育创新团队项目（2022A007）；广西中医药大学桂派杏林拔尖人才资助项目（2022C008）；2025年广西向海经济人才培养支持专项（2025XHRC27)。 |
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| Diversity and antibacterial activity of culturable bacteria isolated from mangrove based on improved in situ culture |
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LI Wangjin1,2,3,LI Mi1,2,3,GAO Mengdie1,2,3,XU Shufen1,2,3,LIU Yonghong1,2,3, GAO Chenghai1,2,3, YI Xiangxi1,2,3*
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（ 1. Institutes of Marine Drugs/Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China; 2. Guangxi Key Laboratory of Marine Drugs/Guangxi University Engineering Research Center of High-Efficient Utilization of Marine Traditional Chinese Medicine Resources, Nanning 530200, China; 3. Beibu Gulf Marine Medicine and Bioproducts Engineering Technology Innovation Center,Naning 530200, China)
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| Abstract: |
| In order to explore marine bacterial resources that exhibit inhibitory activity against human pathogenic bacteria. A improved in situ cultivation technique was implemented, markedly increasing the isolation of culturable bacterial from the rhizosphere soil of ten mangrove plants growing along the Beibu Gulf. The 16S rRNA gene was sequenced to assign taxonomic identity to bacterial isolates, and their antimicrobial potential was assessed with the filter-paper disk diffusion assay. The results were as follows: (1) A total of 240 species assigned to 95 genera, 54 families, 27 orders, 8 classes, and 4 phyla were recovered, among which Streptomyces dominated, comprising 26.7 % of all isolates. (2) Based on 16S rRNA gene comparisons, 13 strains may represent novel species, including strain Photobacterium lucens GXIMD 04217, which exhibited the sequence similarity of 92.94% and may represent a potential new genus. (3) Furthermore, antimicrobial screening identified 25 Actinomycete strains that significantly antimicrobial activity against at least one pathogenic bacterium. Notably, GXIMD 04328 and GXIMD 04558 exhibited significant inhibitory activity against methicillin-resistant Staphylococcus aureus and Talaromyces marneffei, with inhibition zone diameters of (23.5±2.5) mm and (21.2±0.4) mm, respectively. (4) Seven of the active strains exhibited broad-spectrum antimicrobial activity and were able to inhibit a range of human pathogenic bacteria. In conclusion, the rhizosphere soils of mangrove in Beibu Gulf harbor abundant microbial resources, and that the improved in situ cultivation is an effective strategy for discovering novel bacterial species and bioactive strains. |
| Key words: mangrove soil, in situ culture device, culturable bacteria of marine, inhibition of human pathogenic bacteria, actinomyces |
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