热形态建成中HY5抑制SAUR1/2/3/4的转录

冯 雨<sup>1; 2; 3</sup>; 刘周莉<sup>1; 2; 3</sup>; 王 昕<sup>1; 2; 3*</sup>

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*热形态建成中HY5抑制SAUR1/2/3/4的转录*
冯雨^1,2,3, 刘周莉^1,2,3, 王昕^1,2,3*
1. 沈阳大学生命科学与工程学院, 沈阳 110044;2. 中国地质调查局东北地质科技创新中心, 沈阳 110000;3. 自然资源部黑土地演化与生态效应重点实验室, 沈阳 110000

摘要:

SAUR(SMALL AUXIN UP RNA)基因家族是促进下胚轴伸长的重要下游基因,然而在高温诱导的下胚轴伸长中SAURs受调控的分子机制尚有很多不明之处。热形态建成被定义为高等植物在最适温度和逆境高温之间的温和高温范围内发生的一系列形态变化。其中,高温诱导的下胚轴伸长是研究最为透彻的一个。该研究以拟南芥Col野生型、hy5突变体、35S::HY5-HA/Col过表达植物和烟草为材料,采用抑制剂NPA处理实验、定量RT-PCR、染色质免疫共沉淀和双荧光素酶报告基因检测方法探索了高温调控SAUR1/2/3/4的分子机制。结果表明:(1)热形态建成信号转导通路中生长素处于HY5(ELONGATED HYPOCOTYL 5)的下游。(2)在20 ℃和29 ℃中,HY5抑制了SAUR1/2/3/4的转录。(3)在常温和高温中,HY5与SAUR1/2/3/4的启动子区染色质含有E-box的部分结合且这些结合被高温所抑制。(4)HY5对SAUR1/2/3/4的调控依然需要生长素的参与。综上认为,高温通过影响HY5与SAUR1/2/3/4启动子染色质的结合强度来调控这4个基因的转录,并且此调控过程需要生长素。该研究为高温调控下胚轴伸长下游基因的分子机制提供了新的见解。

关键词: 热形态建成, 高温诱导的下胚轴伸长, HY5, SAURs, 转录调控

DOI：10.11931/guihaia.gxzw202410006

分类号:Q943

文章编号:1000-3142(2025)08-1496-12

Fund project:辽宁省“兴辽英才计划”项目(XLYC2203070); 辽宁省科技计划联合计划自然科学基金-面上项目(2024-MSLH-506); 中国地质调查局东北地质科技创新中心区创基金(QCJJ2022-44); 辽宁省博士科研启动基金(201601216)。

HY5 inhibits the transcription of SAUR1/2/3/4 in thermomorphogenesis

FENG Yu^1,2,3, LIU ZhouLi^1,2,3, WANG Xin^1,2,3*

1. College of Life Science and Engineering, Shenyang University, Shenyang 110044, China;2. Northeast Geological S &3.T Innovation Center of China Geological Survey, Shenyang 110000, China;4.3. Key Laboratory of Black Soil Evolution and Ecological Effect, Ministry of Natural Resources, Shenyang 110000, China

Abstract:

The SAUR (SMALL AUXIN UP RNA)gene family is an important downstream gene family that promotes hypocotyl elongation. However, the molecular mechanism by which SAURs are regulated in high temperature induced-hypocotyl elongation remains unclear. Thermomorphogenesis is defined as a series of morphological changes that occur in the mild high temperature range between the optimal temperature and the adverse high temperature in higher plants. Hypocotyl elongation is one of the most intensively researched one. In this study, Arabidopsis Col wild type, hy5 mutant, 35S::HY5-HA/Col overexpressed plants and tobacco were used as research materials. Inhibitor NPA(N-1-naphthylphthalamic acid)treatment, quantitative RT-PCR, chromatin immunoprecipitation and double luciferase reporter gene detection were used to explore the molecular mechanisms of high temperature regulated SAUR1/2/3/4. The results were as follows:(1)Auxin was downstream of HY5(ELONGATED HYPOCOTYL 5)in the thermomorphogenesis signaling pathway.(2)HY5 inhibited the transcription of SAUR1/2/3/4 in 20 ℃ and 29 ℃.(3)At normal temperature and high temperature, HY5 binded to the E-box-containing regions of SAUR1/2/3/4 promoter chromatin, and these bindings were inhibited by high temperature.(4)Auxin was required for the regulation of SAUR1/2/3/4 by HY5. In summary, high temperature regulates the transcription of these four genes by affecting the binding strength of HY5 and SAUR1/2/3/4 promoter chromatin, and this regulatory process requires auxin. This study provides new insights into the molecular mechanisms of hypocotyl elongation downstream genes regulated by high temperature.

Key words: thermomorphogenesis, high temperature-induced hypocotyl elongation, HY5, SAURs, transcription regulation