贵州天名精化学成分的分离与鉴定

Separation and identification of chemical constituents of Carpesium faberi

关键词:  贵州天名精, 天名精, 化学成分, 分离纯化, 结构鉴定, 新化合物

Key words:  Carpesium faberi, Carpesium, chemical constituent, separation and purification, structural identification, new compound

摘要:

为探究贵州天名精(Carpesium faberi)植物的化学成分,该研究采用硅胶柱层析、Rp-C₁₈柱层析及制备型高效液相色谱仪(HPLC)等手段对其进行分离和纯化,并通过理化性质和波谱数据鉴定化合物结构。结果表明:从贵州天名精全草乙酸乙酯部位浸膏中分离得到11个单体化合物,分别鉴定为4α,10α-二羟基-1β(H),5β(H)-11(13)-烯-8,12-愈创木酚(1)、吐叶醇(2)、蚱蜢酮(3)、carpespene F(4)、4-[(1R,2S)-3,3-二甲基-2-(3-氧代丁基)环丁基]正戊基-4-烯-1-乙酸叶醇酯(5)、carpespene I(6)、4β,10β-二羟基-5α(H)-1,11(13)-二烯-8α,12-愈创木酚(7)、木犀草素(8)、(+)-丁香脂素-O-β-D-葡萄糖苷(9)、2-甲氧基-4-(2-丙烯基)苯基β-D-吡喃葡萄糖苷(10)、山奈酚3-O-β-D-葡萄糖苷(11)。其中,化合物5为新化合物,化合物1、4、6、7为愈创木型倍半萜,化合物9、11为首次从该植物中分离得到。该研究结果为贵州天名精的化学成分研究提供了理论依据。

Abstract:

To explore the chemical constituents of Carpesium faberi, the compounds were separated and purified by silica gel column chromatography, Rp-C₁₈ column chromatography and preparative high performance liquid chromatography(HPLC), and their structures were identified by physicochemical properties and spectrum data. The results were as follows: Eleven monomer compounds were isolated from the extract of C. faberi whole grass ethyl acetate and identified as 4α,10α-dihydroxy-1β(H),5β(H)-guai-11(13)-en-8,12-olide(1), vomifoliol(2), grasshopper ketone(3), carpespene F(4), 4-[(1R,2S)-3,3-dimethyl-2-(3-oxobutyl)cyclobutyl] pent-4-en-1-yl acetate(5), carpespene I(6), 4β, 10β-dihydroxy-5α(H)-1,11(13)-guaidien-8α,12-olide(7), luteolin(8),(+)-syringaresinol-O-β-D-glucopyranoside(9), 2-methoxy-4-(2-propenyl)phenyl β-D-glucopyranoside(10), kaempferol 3-O-β-D-glucopyranoside(11). Compound 5 was a new compound, compounds 1, 4, 6, 7 were guaiane-type sesquiterpenoids, and compounds 9, 11 were isolated from this plant for the first time. The research results provide a theoretical basis for the chemical constituent of C. faberi.