| 摘要: |
| 多裂骆驼蓬为西北荒漠地区常见植物,具有抗风固沙、防止水土流失、抑菌杀虫和抗肿瘤等功效。为了增加骆驼蓬属植物开发利用的强度,弥补其基因功能、代谢通路等分子生物学研究层面的空缺,该文利用Illumina高通量测序平台对多裂骆驼蓬叶片进行转录组测序,根据测序结果进行转录组数据拼接、功能注释、序列水平和表达水平等分析。结果表明:共获得7723653900bp核苷酸序列信息,拼接组装得到78641条Unigene,预测出55535条CDS序列。与多个数据库对比后,获得33184个NR数据库注释信息;31835个GO数据库注释信息;17206个KOG数据库Unigene功能分类信息;7617个KEGG数据库代谢通路注释信息。对单核苷酸多态性位点和微卫星信息进行检测分析,共发现86113个SNP位点,6987个SSR信息。该研究首次获得并分析了多裂骆驼蓬的转录组数据,通过基因比对、CDS预测、通路注释、SNP检测和SSR检测等方法,对该植物的基因、通路以及分子标记等方面有了初步的认识,为本种植物后续研究及资源的开发利用奠定了基础。 |
| 关键词: 多裂骆驼蓬,转录组分析,基因注释,SNP,SSR |
| DOI：10.11931/guihaia.gxzw201909063 |
| 分类号:Q943 |
| 文章编号:1000-3142(2021)04-0503-11 |
| Fund project:国家自然科学基金(31110103911);青海省应用基础研究计划项目(2019-ZJ-7042)[SupportedbytheNationalNaturalScienceFoundationofChina(31110103911);BasicResearchProgramofQinghaiProvince(2019-ZJ-7042)]。 |
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| Transcriptome analysis for leaves of Peganum multisectum |
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XIA Mingze1,2, ZHANG Yu1,2, YU Jingya1,2, ZHANG Faqi1*
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1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences,
Xining 810001, China;2. College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Peganum multisectum is a common plant in arid-desert areas of Northwest China, which has the effects of resisting wind and sand fixation, preventing soil erosion, inhibiting bacteria and insects, and resisting tumor. But the lack of molecular biology research on this plant, such as gene function and metabolic pathway, leads to its underutilization. Here in this study, the transcriptomes of P. multisectum leaves were sequenced by the platform of Illumina. According to the sequencing results, we analyzed splicing, functional annotation, sequence level and expression level of the transcriptome data. Then we obtained a total of 7 723 653 900 bp nucleotide sequence information, assembled 78 641 Unigene sequences and predicted 55 535 CDS sequences. After comparing with several databases, 33 184 NR database annotation information, 31 835 GO database annotation information, 17 206 KOG database Unigene functional classification information and 7 617 KEGG database metabolic pathway annotation information were obtained. In addition, we detected a total of 86 113 single nucleotide polymorphism sites and 6 987 microsatellite information. In this study, transcriptome data of P. multisectum were obtained and analyzed for the first time. These analyses not only shed light on the molecular information of P. multisectum, but also provide a certain reference for the post research, development and utilization of this plant. |
| Key words: Peganum multisectum, transcriptome analysis, gene annotation, SNP, SSR |
