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| 茉莉酸甲酯对广藿香JA信号转导途径及
倍半萜合成途径关键基因表达的影响 |
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邓文静1,张宏意1,2,3,欧晓华1,卢昌华1,黄伟展1,严寒静1,2,3*
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1.广东药科大学中药学院,广州510006;2.国家中医药管理局岭南药材生产与开发重点研究室,
广州510006;3.中药材国家现代农业产业技术体系广州综合试验站,广州510006
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| 摘要: |
| 分析外源性茉莉酸甲酯对广藿香JA信号转导途径关键基因JAZ2、MYC2、COI1及倍半萜合成途径关键基因PTS、FPPS、SQLE表达的影响,为深入研究茉莉酸甲酯调控广藿香JA信号转导途径及倍半萜合成途径的分子机制奠定基础。该文分别用0.10和0.25mmol·L-1的MeJA喷施广藿香叶片,于处理后的0、2、6、12、24、48、72h摘取叶片,运用实时荧光定量PCR对JAZ2、MYC2、COI1、PTS、FPPS、SQLE基因的表达量进行检测。结果表明:0.10和0.25mmol·L-1的MeJA对广藿香JA信号转导途径JAZ2、MYC2、COI1及倍半萜合成途径PTS、FPPS、SQLE基因表达均有不同程度的促进作用,其中对JAZ2基因表达影响最显著。0.10mmol·L-1MeJA溶液处理2h时,JAZ2表达量上调13.52倍;0.25mmol·L-1MeJA溶液处理48h时,JAZ2表达量上调19.09倍。JA信号转导途径关键基因JAZ2与倍半萜合成途径关键基因FPPS存在极显著正相关关系。综上结果表明MeJA溶液可诱导广藿香JAZ2、MYC2、COI1、PTS、FPPS、SQLE基因的表达,且不同浓度MeJA对基因表达有着不一样的影响;JAZ2是JA信号转导途径里响应MeJA诱导的主要基因,其可激活倍半萜合成途径FPPS基因的协同表达,进而影响广藿香醇等倍半萜合成。 |
| 关键词: 广藿香,茉莉酸甲酯,JA信号转导途径,倍半萜合成途径,基因表达 |
| DOI：10.11931/guihaia.gxzw202005056 |
| 分类号:Q943 |
| 文章编号:1000-3142(2021)04-0559-08 |
| Fund project:国家自然科学基金(81773829);广东省科技项目(2017A030303081,2017A030303082)[SupportedbytheNationalNaturalScienceFoundationofChina(81773829);ScienceandTechnologyProgramofGuangdongProvince(2017A030303081,2017A030303082)]。 |
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| Effects of MeJA treatment on key genes involved in JA signal transduction and biosynthesis pathway of sesquiterpene in Pogostemon cablin |
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DENG Wenjing1, ZHANG Hongyi1,2,3, OU Xiaohua1, LU Changhua1,
HUANG Weizhan1, YAN Hanjing1,2,3*
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1. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;2. Key Laboratory of State
Administration of Traditional Chinese Medicine or Production &3.Development of Cantonese Medicinal Materials, Guangzhou 510006, China;4.3. Comprehensive Experimental Station of Guangzhou, Chinese Materia Medica, China Agriculture Research System, Guangzhou 510006, China
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| Abstract: |
| The effects of exogenous methyl jasmonate(MeJA)on the key genes(PTS, FPPS, SQLE)expressions of Pogostemon cablin for the JA signal transduction and sesquiterpene synthesis pathways were studies. This would establish a foundation of the molecular mechanisms of MeJA in these two pathways. We treated the leaves of patchouli with 0.10 and 0.25 mmol·L-1 MeJA respectively and picked them at 0、2、6、12、24、48、72 h after treatments. We used qRT-PCR to detect the key genes expression of JAZ2、MYC2、COI1、PTS、FPPS、SQLE. The results were as follows: 0.10 and 0.25 mmol·L-1 MeJA promoted the expression of these genes in different degrees, with the most significant effect on JAZ2. The expression levels of JAZ2 increased 13.52-fold after 2 h at 0.10 mmol·L-1 MeJA treatment and 19.09-fold after 48 h at 0.25 mmol·L-1 MeJA treatment. There was a significant positive correlation between the key gene JAZ2 in JA signal transduction pathway and FPPS in sesquiterpene synthesis pathway. The above results demonstrated that MeJA promoted the expression of JAZ2, MYC2, COI1, PTS, FPPS, SQLE, and different concentrations of MeJA had different effects on gene expression. JAZ2 was the main gene induced by MeJA in JA signal transduction pathway, which can activate the co-expression of FPPS gene in sesquiterpene synthesis pathway, so as to affect the synthesis of sesquiterpene such as patchouli alcohol. |
| Key words: Pogostemon cablin, methyl jasmonate, JA signal transduction pathways, sesquiterpene synthesis pathways, gene expression |
