| 摘要: |
| 为了分析水烛香蒲花粉和茎叶中黄酮类成分,以及不同生长期、不同部位中香蒲新苷、异鼠李素-3-O-新橙皮苷和异鼠李素含量。该文采用UPLC-Q-TOF-MS进行定性分析,结合HPLC法对水烛香蒲不同部位中黄酮类化合物含量进行分析评价,色谱条件为PRAZIS C18柱(250 mm × 4.6 mm,5 μm),以乙腈-0.05%磷酸为流动相,梯度洗脱,体积流量1.0 mL·min-1,检测波长254 nm,柱温30 ℃。结果表明:(1)花粉及茎叶中共解析出42个化合物,其中8个化合物在不同部位中有差异。(2)同一生长环境的水烛香蒲不同生长时期、不同部位中3种黄酮类化合物的含量差异较大。(3)异鼠李素-3-O-新橙皮苷及异鼠李素在香蒲的发育生长过程中含量呈先增加后下降的趋势,茎叶内的这两种成分含量下降明显; 在花粉中,香蒲新苷和异鼠李素-3-O-新橙皮苷含量急剧上升,一周内达到峰值; 茎叶中香蒲新苷含量较低,在大多数样品中未检出。该研究所建立的测定方法简便、重现性好,并对水烛香蒲中黄酮类成分的含量变化规律进行了研究,对花粉及茎叶中化合物差异进行了分析,为蒲黄的合理采收及香蒲植物的资源化利用提供了科学依据。 |
| 关键词: 水烛香蒲, 香蒲新苷, 异鼠李素-3-O-新橙皮苷, 异鼠李素, 动态分析 |
| DOI：10.11931/guihaia.gxzw202005005 |
| 分类号:Q946 |
| 文章编号:1000-3142(2021)05-0831-12 |
| Fund project:国家自然科学基金面上项目(81873001); 中医药公共卫生服务补助专项“全国中药资源普查项目”(财社〔2018〕43号); 财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21)[Supported by the National Natural Science Foundation of China(81873001); Administration of Subsidy Funds for Public Health Services of traditional Chinese Medicine “National Survey of Traditional Chinese Medicine Resources”(Ministry of Finance〔2018〕43); China Agriculture Research System of MOF and MARA(CARS-21)]。 |
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| Dynamic analysis of flavonoids in different parts of Typha angustifolia |
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CHAO Lei1,2, GAO Mingliang2, CAO Yudan2, CHEN Peidong2, YAN Hui2*
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1. Affiliated Hospital, Nanjing University of Chinese Medicine, Nanjing 210029, China;2. School of Pharmacy,
Nanjing University of Chinese Medicine, Nanjing 210023, China
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| Abstract: |
| Inorder to analyze flavonoids in pollen, stem and leaf of Typha angustifolia and the contents of typhaneoside, isorhamnetin-3-O-neohesperidoside and isorhamnetin in different parts of growth periods. UPLC-Q-TOF-MS was used for qualitative analysis, combined with HPLC to analyze and evaluate the contents of flavonoids in different parts of T. angustifolia. A RPAZIS C18 column(250 mm × 4.6 mm, 5 μm)was adopted. The mobile phase was composed of acetonitrile-0.05% phosphoric acid with a gradient elution. The flow rate was 1.0 mL·min-1, the detection wavelength was 254 nm, and the column temperature was 30 ℃. The results were as follows:(1)A total of 42 compounds were identified in pollen, stem and leaf, and eight compounds with great differences in different parts.(2)The contents of three flavonoids in different parts of T. angustifolia during different growth periods in the same growth environment were significantly different.(3)The contents of isorhamnetin-3-O-neohesperidoside and isorhamnetin in the plant were increased first and then decreased, while decreased significantly in the stem and leaf; In pollen, the contents of typhaneoside and isorhamnetin-3-O-neohesperidoside increased quickly and reached a peak within a week; The contents of isorhamnetin in stem and leaf were low and were not detected in most samples. This established method in this study was simple and reproducible. The changes regularity of the flavonoids content in T. angustifolia had been studied and the differences of compounds in pollen, stem and leaf were also analyzed. This research provides a scientific basis for reasonable resource utilization of T. angustifolia. |
| Key words: Typha angustifolia, typhaneoside, isorhamnetin-3-O-neohesperidoside, isorhamnetin, dynamic analysis |
