小果南烛三萜类化学成分研究

Triterpenoids from Lyonia ovalifolia var. elliptica

关键词:  小果南烛, 化学成分, 三萜, 提取分离, 结构鉴定

Key words:  Lyonia ovalifolia var. elliptica, chemical constituents, triterpenes, extraction and isolation, structure identification

摘要:

羊毛甾烷三萜是南烛属植物主要活性成分之一。为进一步研究南烛属植物功效物质基础,结合民族民间对小果南烛的应用实际,该文利用硅胶、MCI、Sephadex LH-20、半制备高效液相色谱等植物化学技术手段对该植物95%乙醇提取物进行分离、纯化,综合理化性质和波谱数据对其化合物进行鉴定。结果表明:从小果南烛的茎、叶提取物中分离得到14个三萜及其皂苷,分别鉴定为mollic acid 3-O-α-L-arabinopyranoside(1)、mollic acid 3-O-β-D-glucopyranoside(2)、cycloart-3,7-dihydroxy-24-en-28-oic acid(3)、白桦脂酸(4)、1β, 3α, 11α-trihydroxy-urs-12-ene(5)、 oleanderolide(6)、(Z)-马斯里酸-3-O-对香豆酸酯(7)、木栓酮(8)、坡模酸(9)、2α, 3α-二羟基乌苏-12-烯-28-酸(10)、科罗索酸(11)、齐墩果酸(12)、熊果酸(13)、委陵菜酸(14)。其中,化合物1-7为首次从南烛属植物分离得到,化合物8-11,14为首次从小果南烛植物中分离得到。

Abstract:

Lanostane triterpene is one of the main active components of Lyonia Nutt. In order to further study the functional material basis of Lyonia, and in combination with the application of ethnic and folk practices to Lyonia ovalifolia var. elliptica, 95% ethanol extract of the plant was treated with phytochemical techniques such as silica gel, MCI, Sephadex LH-20, HPLC, fourteen lanostane triterpenes and their glycosides were identified by the physical and chemical properties. These fourteen compounds were identified as mollic acid 3-O-α-L-arabinopyranoside(1), mollic acid 3-O-β-D-glucopyranoside(2), cycloart-3,7-dihydroxy-24-en-28-oic acid(3), betulinicacid(4), 1β, 3α, 11α-trihydroxy-urs-12-ene(5), oleanderolide(6), 3-O-cis-p-coumaroyl maslinic acid(7), friedelin(8), pomolic acid(9), 2α, 3α-dihydroxy-urs-12-en-28-oic acid(10), corosolic acid(11), oleanolic acid(12), ursolic acid(13), tormentic acid(14). Compounds 1-7 were isolated from this genus for the first time, and compounds 8-11, 14 are isolated from this plant for the first time.