| 摘要: |
| 为观察大血藤醇提物抗炎、镇痛、止血活性,该文采用75%乙醇提取制备大血藤醇提物(AESC),利用HPLC法测定其绿原酸含量; KM鼠(或新西兰兔)在测定抗炎、镇痛、止血活性时随机分为空白对照组、阳性对照组(云南白药酊组)、AESC组,依次测定其抑制二甲苯致小鼠耳肿胀度作用、痛阈值和兔肝脏局部创面损伤出血的记分分值,分别考察其抗炎、镇痛、止血作用。结果表明:AESC中绿原酸含量为(0.294 ± 0.013 5)%; 与空白组比较,剂量为0.700 g·kg-1 的AESC组能显著减轻二甲苯所致的小鼠耳肿胀度(P<0.01),抑制率达26.3%; 与空白组及给药前比较,剂量为1.40 g·kg-1 的AESC组均能显著提高小鼠痛阈值(P<0.01); 与空白组相比,剂量为1.40 g·kg-1的 AESC组能显著提高兔肝脏局部创面损伤出血的记分分值(P<0.001)。大血藤醇提取物具有显著的抗炎、镇痛、止血作用,有望将其开发为抗炎、镇痛、止血制剂。该结果也为大血藤的临床应用提供理论依据。 |
| 关键词: 大血藤, 抗炎活性, 镇痛活性, 止血活性, 绿原酸 |
| DOI：10.11931/guihaia.gxzw202003023 |
| 分类号:Q946; R932 |
| 文章编号:1000-3142(2021)07-1120-06 |
| Fund project:国家自然科学基金(21977021)[Supported by the National Natural Science Foundation of China(21977021)]。 |
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| Preliminary study on anti-inflammatory, analgesia and hemostasis effects of alcohol extracts from Sargentodoxa cuneata |
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LIAO Na1,2,3, HUANG Guangwei1, TANG Hongyan1, ZHOU Chuanjian1,
YANG Weihao1, WANG Hengshan2*
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1. Liuzhou LMZ Co., Ltd., Liuzhou 545001, Guangxi, China;2. State Key Laboratory for Chemistry and Molecular Engineering of Medicinal
Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China;3.
3. Medical School, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, China
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| Abstract: |
| To investigate the anti-inflammatory, analgesia and hemostasis effects of the 75% alcohol extract from Sargentodoxa cuneata(AESC). The AESC was prepared by ethanol extraction, and its chlorogenic acid content was determined by HPLC. KM mice(or New Zealand rabbits)were randomly divided into the blank group, positive control group(Yunnan Baiyao Tincture group)and the AESC group in determination of anti-inflammatory, analgesia and hemostasis activities. The anti-inflammatory, analgesic and hemostasis effects were investigated through determining xylene-induced ear swelling of mice, pain threshold and rabbit liver bleeding model. The results were as follows: The content of chlorogenic acid was(0.294 ± 0.013 5)%; Compared with the blank group, AESC group(Dose 0.700 g·kg-1)could significantly reduce the ear swelling induced by xylene(P < 0.01), and the inhibition rate was 26.3%; Compared with the blank group and before administration, AESC group(Dose 1.40 g·kg-1)could significantly increase the pain threshold of mice(P<0.01); Compared with the blank group, AESC group(Dose 1.40 g·kg-1)could significantly improve the score of local wound bleeding(P<0.001). The alcohol extracts of S. cuneata showed significant effects in anti-inflammatory, analgesic and hemostasis, could be developed as the anti-inflammatory, analgesic, hemostatic agents. And this study will provides theoretical basis for clinical application of the S. cuneata. |
| Key words: Sargentodoxa cuneata, anti-inflammatory activity, analgesia activity, hemostasis activity, chlorogenic acid |
