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| 中粒咖啡WRKY基因家族的生物信息学分析 |
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付 春1,2*, 唐 雪1,2, 杨瑶君1,2, 江 纳3
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1. 乐山师范学院 竹类病虫防控与资源开发四川省重点实验室, 四川 乐山, 614000;2. 乐山师范学院 生命科学学院,
四川 乐山, 614000;3. 乐山师范学院 数学与信息科学学院, 四川 乐山, 614000
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| 摘要: |
| WRKY转录因子是植物信号网络中不可缺少的部分,作为植物中最大的转录因子家族之一,在植物的多种应激反应中发挥着重要作用。该文利用生物信息学方法对中粒咖啡WRKY蛋白家族的理化特性及其分子进化进行了详细分析。结果表明:(1)CcWRKY蛋白氨基酸数量在103～994个之间,均无信号肽,推测其为非分泌性蛋白; 其二级结构以无规则卷曲为最主要的结构元件,三级结构主要分为6类,其中以CcWRKY15、CcWRKY25、CcWRKY37和CcWRKY42为主要成员的D类结构最稳定。(2)保守结构域及进化树分析结果显示,中粒咖啡WRKY基因家族含有49个成员,其中的10个成员归为WRKY第Ⅰ家族,34个成员归为WRKY第Ⅱ家族,5个成员归为WRKY第Ⅲ家族。(3)中粒咖啡 WRKY47基因与其他物种的系统进化分析结果显示,WRKY47与烟草亲缘关系最近,与非洲油棕(Elaeis guineensis)亲缘关系最远,说明WRKY47蛋白在生物进化过程中比较保守。该研究结果可为中粒咖啡WRKY基因家族分子功能的深层次研究提供一定的借鉴作用,对进一步探究中粒咖啡WRKY基因的功能、进化以及分子育种具有重要意义。 |
| 关键词: 中粒咖啡, WRKY, 转录因子, 系统进化, 生物信息学 |
| DOI：10.11931/guihaia.gxzw202003075 |
| 分类号:Q943.2 |
| 文章编号:1000-3142(2021)11-1905-15 |
| Fund project:国家自然科学基金(31470655); 四川省科技厅项目(17ZZ019); 乐山市科技局重点研究项目(18JZD101); 乐山师范学院人才启动项目(XJR17005); 乐山师范学院重点研究项目(LZD010); 乐山师范学院横向项目(LHX190786)[Supported by the National Natural Science Foundation of China(31470655); Sichuan Provincial Science and Technology Department Project(17ZZ019); Key Research Project of Leshan Municipal Science and Technology Bureau(18JZD101); Leshan Normal University Talent Launch Project(XJR17005); Key Research Project of Leshan Normal University(LZD010); Cooperative Project of Leshan Normal University(LHX190786)]。 |
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| Bioinformatics analysis of WRKY gene family in Coffea canephora |
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FU Chun1,2*, TANG Xue1,2, YANG Yaojun1,2, JIANG Na 3
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1.1. Bamboo Disease and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal University, Leshan
614000, Sichuan, China;2.2. College of Life Science, Leshan Normal University, Leshan 614000, Sichuan, China;3.3. College of
Mathematics and Information Science, Leshan Normal University, Leshan 614000, Sichuan, China
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| Abstract: |
| WRKY transcription factor is an indispensable part of plant signal network. As one of the largest family of transcription factors in plants, WRKY transcription factor plays an important role in various stress responses of plants. The physicochemical properties and molecular evolution of WRKY protein family of Coffea canephora were analyzed in detail by bioinformatics methods. The results were as follows:(1)The amino acids numbers of WRKY proteins were from 103 to 994, and there was no signal peptide, and were presumed to be a non-secretory protein. Random coil was the most important structural element in the secondary structure of its WRKY proteins. The tertiary structure of WRKY proteins were mainly divided into six categories, among which the D-class structure with CcWRKY15、CcWRKY25、CcWRKY37 and CcWRKY42 as the main members was the most stable.(2)Conserved domain and phylogenetic tree analysis indicated that the WRKY gene family in C. canephora contained 49 members, 10 of which were classified as WRKY class I, 34 members were classified as WRKY class Ⅱ, and 5 members were classified as WRKY class Ⅲ.(3)The phylogenetic analysis of WRKY47 gene of C. canephora and other species showed that WRKY47 of C. canephora was the most closely related to tobacco and the most distant to Elaeis guineensis, indicating that WRKY47 protein is relatively conservative in the process of biological evolution. The results of this study can provide some reference for the further study of the molecular function of WRKY gene family of C. canephora, and have important practical significance for further exploration of the function, evolution and molecular breeding of WRKY gene in C. canephora. |
| Key words: Coffea canephora, WRKY, transcription factor, phylogenetic evolution, bioinformatics |
