不同种源花榈木苗期生长及生理特性比较

Comparison of growth and physiological characteristics of Ormosia henryi seedlings from different provenances

关键词:  花榈木, 种源, 生长差异, 生理, 主成分分析

Key words:  Ormosia henryi, provenance, growth difference, physiological, principal component analysis

摘要:

为了解不同种源花榈木在贵阳的生长特性和差异,该文通过对10个种源地花榈木进行育苗试验,测定其两年生实生苗的苗高、地径、生物量、叶片光合参数、光合色素、硝酸还原酶活性、硝态氮含量和根系活力,并进行差异性分析。结果表明:(1)10个种源花榈木净光合速率、气孔导度、胞间CO₂浓度、蒸腾速率和水分利用效率差异显著(P<0.05),表明不同种源花榈木光合特性及光能利用效率具有较大差异,浙江杭州和浙江永康花榈木是具有较高光合生长潜力的种源。(2)种源间的叶绿素含量、硝酸还原酶、硝态氮、根系活力存在显著差异,福建建瓯种源的叶绿素a、叶绿素b含量和叶绿素总量最高,能够将光合原初反应过程中积蓄的光能进行高效地传递,促进碳的同化; 贵州花溪种源硝酸还原酶活性最大,硝态氮含量最高,对氮元素的利用能力较强,能够促进植物蛋白质、氨基酸和叶绿素等的合成; 贵州望谟种源根系活力最大,吸收养分的能力强。(3)各种源间苗高、地径和生物量的分配存在显著差异,浙江杭州种源的植株枝叶繁茂、根系发达,生长表现好,安徽黄山种源的植株矮小,生长表现较差; 浙江杭州种源将生物量更多分配在根和叶,提高其根系吸收养分和叶片获取光能的能力,安徽黄山种源总体生物量积累最少,长势最差。(4)通过主成分分析法对各种源的花榈木适应性进行综合评价,结果显示浙江杭州种源>贵州黎平种源>浙江永康种源>贵州望谟种源>福建建瓯种源>贵州凯里种源>贵州石阡种源>贵州花溪种源>贵州平塘种源>安徽黄山种源。综上结果表明,浙江杭州、贵州黎平和浙江永康种源花榈木对贵阳地区立地环境具有较强的适应能力和生长潜力。

Abstract:

In order to understand the growth characteristics and differences of different Ormosia henryi provenances in Guiyang, we collected seeds from ten different provenances of O. henryi and conducted seedling experiments. We compared differences in seedling height, root collar diameter, biomass, leaf photosynthetic parameters, photosynthetic pigment, activity of nitrate reductase, content of nitrate nitrogen and the root activity of the biannual seedlings, and then analyzed the differences in ten provenances of O. henryi. The results were as follows:(1)There were significant differences in the net photosynthetic rate(P_n), stomatal conductance(G_s), intercellular carbon dioxide concentration(C_i), transpiration rate(T_r)and water use efficiency(WUE)among ten O. henryi provenances(P<0.05), indicating that different O. henryi provenances showed significant differences in photosynthetic characteristics and light use efficiency. Plants from Hangzhou and Yongkang of Zhejiang had the higher photosynthetic growth potential.(2)There were significant differences in chlorophyll content, nitrate reductase, nitrate nitrogen and root activity among provenances. The contents of chlorophyll a and chlorophyll b and total content of chlorophyll were the highest in Jian'ou of Fujian provenance. These plants could efficiently transfer the accumulated light energy during the initial reaction of photosynthesis and promote carbon assimilation. Huaxi of Guizhou provenance had the highest nitrate reductase activity, the highest nitrate nitrogen content, and strong utilization ability of nitrogen elements, which could promote the synthesis of plant protein, amino acid and chlorophyll. The root system from plants in the Wangmo of Guizhou provenance had the greatest activity and the ability to absorb nutrients.(3)There were significant differences in the distribution of seedling height, ground diameter and biomass among all provenances. The plants from Hangzhou of Zhejiang provenance had luxuriant branches and leaves, developed roots and good growth performance, while the plants from Huangshan of Anhui provenance were short and had poor growth performance. Hangzhou of Zhejiang provenance distributed more biomass in roots and leaves to increase the ability of roots to absorb nutrients and leaves to acquire light energy. Huangshan of Anhui provenance accumulated the least biomass and had the worst growth.(4)The adaptability of ten provenances of O. henryi was comprehensively evaluated by principal component analysis. The results showed that Hangzhou of Zhejiang provenance > Liping of Guizhou provenance > Yongkang of Zhejiang provenance > Wangmo of Guizhou provenance > Jian'ou of Fujian provenance > Kaili of Guizhou provenance > Shiqian of Guizhou provenance > Hauxi of Guizhou provenance > Pingtang of Guizhou provenance > Huangshan of Anhui provenance. In conclusion, Plants from Hangzhou of Zhejiang, Liping of Guizhou and Yongkang of Zhejiang O. henryi provenances had high potential for adaptation and growth potential in Guiyang.