| 摘要: |
| 现有主流猕猴桃品种的遗传背景相对单一，亲本来源地理分布狭窄，亲缘关系不清晰。 为充分利 用杂种优势，该研究以广西植物研究所猕猴桃种质资源圃收集的53个猕猴桃品种(品系)叶片为材料，使用SCoT分子标记进行遗传多样性分析。 结果表明:(1)10条引物在53份猕猴桃供试材料中共扩增出110条条带，各引物扩增的条带在8~15条之间，引物平均扩增条带数为11条；其中多态性条带101条，引物平均 扩增多态性条带数为10.1条，多态性比例为91.81%。 (2)聚类分析显示猕猴桃品种(品系)没有按类型、倍 性或选育地等形成明显有规律的聚类关系。 但相对来说，同一杂交后代个体之间的亲缘关系比亲本与后代 个体之间的亲缘关系更近；芽变品种与原品种并没有表现出特别近的遗传距离，说明芽变材料的突变可能 在基因组或染色体层面发生了较大范围的重组、复制或丢失；‘楚红’‘桂红’‘湘吉红’和‘龙藏红’4个红肉 品种与‘红阳’亲缘关系明显较远，说明其可能由不同亲本衍生而来；初步验证了 ‘桂海四号’可能为 ‘HoRT16’亲本之一的推测。 |
| 关键词: 猕猴桃品种, 中华猕猴桃, 亲缘关系, SCoT分子标记 |
| DOI：10.11931/guihaia.gxzw202006039 |
| 分类号: |
| 文章编号:1000-3142(2022)01-0049-09 |
| Fund project:国家自然科学基金(32060643,32060666); 现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-13-1); 广西科技重大专项(桂科AA17204097-10); 广西植物研究所基本业务费(桂植业21008)[Supported by the National Natural Science Foundation of China(32060643, 32060666); Major Projects of Guangxi Innovation Team Building in the State Modern Agricultural Induantry Technology System(nycytxgxcxtd-13-1); Major Projects of Guangxi Science and Technology(AA17204097-10); Fundamental Research Fund of Guangxi Institute of Botany(21008)]。 |
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| The SCoT analyses of genetic relationshipof 53 kiwifruit cultivars(lines) |
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QI Beibei, WANG Faming, MO Quanhui, YE Kaiyu, GONG Hongjuan,
LIU Pingping, JIANG Qiaosheng, LI Jiewei*
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Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Zhuang Autonomous Region
and Chinese Academy of Sciences, Guangxi Institute of Botany, Guilin 541006, Guangxi, China
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| Abstract: |
| The genetic backgrounds of the popular kiwifruit varieties are relatively homogeneous, the range of geographical distribution of parental origin is narrow, and the genetic relationships are unclear. In order to take full advantage of the heterosis, the leaves of 53 kiwifruit cultivars(lines)from the germplasm resource garden of Guangxi Institute of Botany were collected, and SCoT molecular markers were used to assess genetic diversity and relationship among the 53 kiwifruit cultivars(lines). The results were as follows:(1)Ten informative primers were selected and a total of 110 bands were produced among the cultivars. A range of 8-15 bands were produced by the selected primer, with an average of 11 bands per primer. Among the 110 bands, there were 101 polymorphic bands, which account for 91.81%.(2)The phyolgenetic relationships were not exactly consistent with the ecological type, DNA ploidy, and geographical distributing according to the cluster analysis. However, the phyolgenetic relationships among the offspring were closer than the parent-offspring convergence. And the phyolgenetic relationships between the sport mutation lines and theirs original cultivars were not so close as we assumed, which suggested that there may be inappropriate recombination, disturbed replication and/or chromosome loss during the budding processes. The 4 cultivars ‘Chuhong' ‘Guihong' ‘Xiangjihong' and ‘Longzanghong' showed relative closer phyolgenetic relationships with the cultivar ‘Hongyang', indicated that it may be derived from different parent. Our results provided a primay validation that ‘Guihaia-4' should be one of the parents of ‘Hort16A'. |
| Key words: kiwifruit cultivars, Actinidia chinensis, genetic relationship, SCoT molecular marker |
