战骨黄酮碳苷类化合物的大孔树脂富集工艺研究

Purification technology of total flavonoid-C-glycosides of Premna fulva by macroporous resin

关键词:  战骨, 黄酮碳苷, 大孔树脂, 正交试验

Key words:  Premna fulva, flavonoid-C-glycosides, macroporous resin, orthogonal test

摘要:

黄酮碳苷,作为战骨植物中的有效成分之一,具有抗骨关节炎的功效。为优化植物战骨茎中总黄酮碳苷的富集纯化工艺,该研究以战骨中5个黄酮碳苷为考察指标,通过对13种大孔树脂的静态吸附与解吸实验,优选出合适的大孔树脂,利用高效液相色谱对结果进行检测,然后利用正交工艺优化富集纯化条件。结果表明:(1)XAD-16N型大孔树脂对植物战骨茎中黄酮碳苷具有较好的吸附和解吸效果,通过单因素筛选及正交工艺优化,最终确定其最佳富集纯化工艺为上样浓度为原提取液稀释2倍、上样流速2 BV·h^-1、洗脱液浓度60%乙醇。(2)总黄酮碳苷的含量由1.73%(原植物中含量)提高到14.50%,提高了7.38倍。以上结果表明,优化的提取工艺稳定可行,筛选的大孔树脂富集纯化效果良好,为植物战骨茎的药物开发提供数据参考。

Abstract:

Flavonoid-C-glycosides, as one kind of the effective components in Premna fulva, have the effect of antigen-induced arthritis. In order to optimize the enrichment and purification process of P. fulva stems, through static adsorption and desorption experiments on 13 kinds of macroporous resins with five flavonoid-C-glycosides in P. fulva stems as the index, a suitable macroporous resin was selected, and the results were tested by the HPLC, and then the orthogonal process was used to optimize the enrichment and purification conditions. The results were as follows:(1)XAD-16N macroporous resin has good adsorption and desorption effects on the five flavonoid -C-glycosides in P. fulva stems. Through single factor screening and orthogonal process optimization, the best enrichment and purification process was finally determined as that the loading concentration was two times the original extract diluted, the loading flow rate was 2 BV·h^-1, and the eluent concentration was 60% ethanol.(2)The contents of five flavonoid-C-glycosides increased from 1.73%(in the original plant)to 14.50%, an increase of 7.38 times. All the results indicate that the selected extraction process is stable and feasible, and the selected macroporous resin has good separation and purification effects, which provides a reference for the drug development of P. fulva stems.