| 摘要: |
| 广藿香药材以广藿香酮含量较高的酮型广藿香为最优质。而广藿香酮为一种萜类成分,其生物合成途径尚未明确。MVA(甲羟戊酸)途径是萜类化合物生物合成的重要途径。为了分析MVA途经基因表达与化学成分的相关性从而获得促进广藿香酮合成的潜在基因,该文以2种酮型广藿香栽培品种(石牌广藿香、高要广藿香)为材料,通过实时定量PCR分析基因表达和主要成分含量测定,并研究了供试材料不同时期的茎、叶中与甲羟戊酸代谢途径相关的HMGR、MK、MDD基因表达及化学成分。结果表明:(1)HMGR基因在石牌广藿香嫩叶中表达更明显; MK基因在石牌广藿香和高要广藿香中表达模式相似,主要在老茎中表达; MDD基因在石牌广藿香叶中比高要广藿香表达量更高,在两种广藿香的茎中表达模式相似。(2)同属于酮型广藿香,石牌广藿香与高要广藿香的化学成分相似,老叶广藿香醇含量最高,老茎的广藿香酮含量更高。(3)MDD和MK基因与广藿香酮的合成正相关。综上结果所述,酮型广藿香两个栽培种MVA途径的基因表达模式相似,MDD和MK基因可能为酮型广藿香萜类代谢途径的关键基因。 |
| 关键词: 广藿香, MVA途径, 广藿香酮, MDD基因, MK基因 |
| DOI：10.11931/guihaia.gxzw202011018 |
| 分类号:Q943 |
| 文章编号:1000-3142(2022)05-0745-08 |
| Fund project:浙江省医药卫生科技计划项目(2019RC270); 宁波市自然科学基金(2019A610372); 广东省科技计划项目(2019B030316019); 广东省自然科学基金(2018A030313255)[Supported by Medical and Health Science and Technology Planning Project of Zhejiang Province(2019RC270); Ningbo Natural Science Foundation(2019A610372); Science and Technology Planning Project of Guangdong Province(2019B030316019); Guangdong Natural Science Foundation(2018A030313255)]。 |
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| Gene expression analysis of MVA pathway in Pogostemon cablin and its correlation with synthesis of main components |
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KANG Dali1, HU Weiming2, OUYANG Puyue3,4*
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1. Ningbo College of Health Sciences, Ningbo 315100, Zhejiang, China;2. Lushan Botanical Garden, Chinese Academy of Sciences,
Jiujiang 510650, Jiangxi, China;3. College of Agriculture, Guangxi University, Nanning 530005, China;4. China
Guangdong Food and Drug Vocational College, Guangzhou 510520, China
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| Abstract: |
| Among all the Pogostemon cablin medicinal materials, the pogostone-type one with higher content of pogostone is regarded the best. However, as a terpenoid component, the biosynthesis of pogostone is still not clear. MVA(cytoplasmic)pathway is an important pathway for terpenoid biosynthesis. In order to clarify the correlation between chemicals and related gene expression, in this study, the gene expression of HMGR, MK and MDD of cytoplasmic pathway and the chemical components in stems and leaves of two pogostone-type P. cablin cultivars(Shipai and Gaoyao)were studied, by using real time quantitative PCR to analyze gene expression and main components measurement. The results were as follows:(1)HMGR gene was more obviously expressed in the tender leaves of Shipai cultivar. The expression pattern of MK gene in Shipai cultivar and Gaoyao cultivar was similar, and MK gene was mainly expressed in old stems. MDD gene was more expressed in Shipai cultivar leaves than that of Gaoyao cultivar, but was expressed in similar patterns in the stems of Shipai cultivar and Gaoyao cultivar.(2)Both belonging to pogostone-type P. cablin, Shipai cultivar and Gaoyao cultivar had similar chemical components. Old leaves had the highest patchoulol content and old stems had the highest pogostone content.(3)MDD and MK genes were positively related to the synthesis of pogostone, which will lay a foundation for exploring key genes in P. cablin terpenoid metabolism pathway. In conclusion, the gene expression patterns of MVA pathway in two P. cablin cultivars are similar, and MDD and MK genes may be the key genes of terpenoid metabolism pathway in P. cablin. |
| Key words: Pogostemon cablin, MVA pathway, pogostone, MDD gene, MK gene |
