| 摘要: |
| 为探索黄花倒水莲春梢生理生化特性的差异以及不同内源激素的变化规律,该文对黄花倒水莲春梢的生长动态进行监测,采用间接酶联免疫吸附法(ELISA)测定脱落酸(ABA)、生长素(IAA)、赤霉素(GA)、乙烯(ETH)和玉米素核苷(ZR)五种内源激素含量的动态变化,并对两者间的相关性进行分析。结果表明:(1)黄花倒水莲春梢生长发育过程可分为快速增长期(0～12 d)、生长转折期(16～20 d)和缓慢增长期(24～32 d)三个阶段。(2)内源激素ABA、GA、ETH和ZR含量在缓慢增长期显著高于快速增长期和生长转折期,IAA含量各时期差异较小。(3)春梢长、底部叶长和叶宽在快速增长期与ABA、GA、ETH和ZR含量呈负相关,且与ZR含量具有一定显著性,与IAA含量呈正相关; 生长转折期,各指标与GA、ETH和ZR含量呈正相关,与GA含量具有一定显著性,与ABA含量呈负相关; 缓慢增长期,各指标与五种内源激素含量均呈正相关,与IAA和ZR含量具有一定显著性。该研究结果为生产上利用外源激素调控黄花倒水莲春梢抽出以及生长提供了理论基础。 |
| 关键词: 春梢, 生长变化, 内源激素, 含量变化, 相关性 |
| DOI：10.11931/guihaia.gxzw202009008 |
| 分类号:Q945; S685 |
| 文章编号:1000-3142(2022)05-0796-06 |
| Fund project:广西科技重大专项(桂科AA18118015); 桂林市重大专项项目(20190101); 桂林市科学研究与技术开发计划项目(20190208-3); 国际植物园保护联盟项目(BGCI 30412); 广西植物功能物质研究与利用重点实验室项目(ZRJJ2018-7); 广西植物研究所基本业务费项目(桂植业19002)[Supported by Guangxi Science and Technology Major Project(Guike AA18118015); Guilin Major Special Project(20190101); Guilin Scientific Research and Technology Development Plan Project(20190208-3); International Botanical Garden Protection Alliance Project(BGCI 30412); Fund of Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization(ZRJJ2018-7); Fundamental Research Foundation of Guangxi Institute of Botany(Guizhiye 19002)]。 |
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| The relationship between the growth of spring shoots and the dynamic changes of endogenous hormones in Polygala fallax |
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ZHANG Yuxian1, TANG Hui2,3, HUANG Xiyang2,3, LIU Baoyu2,3, LI Wenlan1*
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1. College of Life Sciences and Technology, Guangxi University/State Key Laboratory for Conservation and Utilization of SubtropicalAgro-bioresources,
Nanning 530004, China;2. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006,
Guangxi, China;3. Guangxi Key Laboratory of Functional Phytochemicals Research andUtilization, Guilin 541006, Guangxi, China
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| Abstract: |
| In order to explore the difference of physiological and biochemical characteristics of spring shoots and the change rule of different endogenous hormones in Polygala fallax. The growth dynamics of spring shoots were monitored, and the contents of abscisic acid(ABA), auxin(IAA), gibberellin(GA), ethylene(ETH)and zeatin nucleoside(ZR)were determined by indirect enzyme-linked immunosorbent assay(ELISA)change. The results were as follows:(1)The growth and development of the spring shoots of P. fallax could be divided into rapid growth period(0-12 d), growth transition period(16-20 d)and slow growth period(24-32 d).(2)The contents of endogenous hormones ABA, GA, ETH and ZR in slow growth period were significantly higher than those in rapid growth period and growth transition period, and the difference of IAA contents in each period was small.(3)The spring shoot length, bottom leaf length and width were negatively correlated with ABA, GA, ETH and ZR contents in the rapid growth period, and were significantly correlated with ZR content, and positively correlated with IAA content; In the growth transition period, each index was positively correlated with GA, ETH and ZR contents, and was significantly correlated with GA content, but negatively correlated with ABA content; During the slow growth period, each index was positively correlated with the contents of five endogenous hormones, and was significantly correlated with the contents of IAA and ZR. The results provide a theoretical basis for the use of exogenous hormones to regulate the spring shoot extraction and growth of P. fallax. |
| Key words: spring shoot, growth change, endogenous hormone, content changes, correlation |
