| 摘要: |
| 硒(Se)为植物非必需营养元素,但适量的硒对植物生长发育具有促进作用,因此研究植物生长对硒的响应及其生理机制具有重要意义。该研究采用盆栽试验,设置6种不同的土壤硒处理(0、5、10、25、50、100 mg·kg-1),观察不同硒处理对白术幼苗生理生长的影响及其综合效应。结果表明:(1)相比于对照处理,5 mg·kg-1的硒处理可以提高白术幼苗鲜重、叶片叶绿素相对含量(SPAD值)以及可溶性糖(SS)含量,增幅分别为8.8%、8.4%和60.3%; 显著降低白术幼苗叶片的过氧化物酶(POD)和过氧化氢酶(CAT)活性; 同时减少叶片可溶性蛋白(SP)和丙二醛(MDA)含量,降幅分别为32.1%和18.9%。(2)25 mg·kg-1以上硒处理则不利于白术幼苗的生长,会导致MDA和超氧阴离子自由基(·O2-)含量显著上升,使白术幼苗的抗氧化系统遭受胁迫。(3)主成分分析表明,鲜重、株高、叶片SP含量、CAT和POD活性等可作为反映白术幼苗生理生长的重要特征指标。综上表明,土施适量硒可以显著促进白术幼苗的生长,并以5 mg·kg-1的硒处理效果最佳,可用于白术幼苗的高效优质繁育和工业化生产。 |
| 关键词: 硒, 白术, 生长, 形态特征, 抗氧化系统 |
| DOI：10.11931/guihaia.gxzw202102015 |
| 分类号:Q945 |
| 文章编号:1000-3142(2022)06-1008-10 |
| Fund project:湖北省重点研发计划项目(2020BCA059); 湖北省农业科技创新中心重大科技研发项目(2020-620-000-002-04); 恩施自治州科技计划项目(D20200017) Symbol〖A@ Supported by Hubei Key Research and Development Program(2020BCA059); Hubei Technology Innovation Center for Agricultural Sciences-Key Research and Development Project of Science and Technology(2020-620-000-002-04); Science and Technology Plan of Enshi Autonomous Prefecture(D20200017) Symbol〗A@ 。 |
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| Effects of different soil selenium levels on growth and partial physiological characteristics of Atractylodes macrocephala seedlings |
|
ZHANG Meide1, WU Haitang1, ZHANG Yajuan2, LI Darong1,
XIONG Linke1, DUAN Yuanyuan1, ZHOU Wuxian1*
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1. Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, Hubei, China;2. Enshi
Tujia and Miao Autonomous Prefecture of Agriculture and Rural Bureau, Enshi 445000, Hubei, China
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| Abstract: |
| Although selenium(Se)has not been considered an essential element for higher plants, but moderate Se plays an important role in promoting plant growth. Therefore, it is of great significance to learn the roles and underlying mechanism of Se in regulating the growth of Atractylodes macrocephala. In this study, a pot experiment was conducted to explore the comprehensive effects of different Se treatments(0, 5, 10, 25, 50, 100 milligram Se per kilogram air-dry soil)on growth of A. macrocephala seedlings. The results were as follows:(1)5 mg·kg-1 Se treatment increased the fresh weight, leaf SPAD value(soil and plant analyzer development value)and leaf soluble sugar(SS)content of A. macrocephala seedlings by 8.8%, 8.4% and 60.3%, respectively; While decreased the leaf soluble protein(SP)and malondialdehyde(MDA)contents by 32.1% and 18.9% respectively, as well as depressed the activities of peroxidase(POD)and catalase(CAT), compared with the control.(2)Treatments of Se >25 mg·kg-1 were not beneficial to the growth of A. macrocephala seedlings, resulting in a significant increase in MDA and superoxide anion radical(·O2-)content, which destroyed the antioxidant system.(3)Principal component analysis showed that fresh weight, plant height, leaf SP content and activities of CAT and POD could be defined as critical indexes to learn the growth condition of A. macrocephala seedlings. In conclusion, the appropriate soil Se levels could significantly promote the growth of A. macrocephala seedlings, and 5 mg·kg-1 soil Se level was the optimum. Application of appropriate Se to the soil could be beneficial for efficient breeding and industrial production of A. macrocephala. |
| Key words: selenium, Atractylodes macrocephala, growth, morphological characteristics, antioxidant system |
