香合欢EST-SSR标记开发及种间通用性研究

EST-SSR marker development and interspecific generality of Albizia odoratissima

关键词:  香合欢, EST-SSR, 分子标记, 通用性, 多态性

Key words:  Albizia odoratissima, EST-SSR, molecular marker, transferability, polymorphism

摘要:

香合欢是我国南方特有的珍贵用材树种。为了对其种质资源开展群体遗传学研究,该研究根据香合欢转录组测序结果设计开发EST-SSR引物,并在黄豆树、南洋楹、黑木相思、格木等近缘树种中进行通用性分析。结果表明:(1)所开发的243对引物有171对能够成功扩增出目的条带,在香合欢、黄豆树、南洋楹、黑木相思、格木中的有效扩增率分别为63.79%、33.75%、45.68%、41.56%、14.81%; 多态性比率分别为23.87%、12.20%、9.01%、3.96%、2.78%; 5个物种间均通用的引物有18对。(2)通过验证共获得香合欢SSR多态性标记37个,黄豆树和南洋楹多态性标记均为10个,黑木相思多态性标记4个,格木多态性标记1个。(3)所开发的香合欢EST-SSR标记,可以满足开展香合欢群体遗传学相关研究的需要,并在黄豆树、南洋楹等近缘树种中具有较好的通用性和研究实用性。综上认为,EST-SSR标记可在香合欢、黄豆树、南洋楹、黑木相思、格木等树种的种质资源遗传多样性评价、育种材料指纹图谱构建、群体交配系统分析等方面提供可靠的研究工具,对香合欢种质资源的保护和利用具有重要意义。

Abstract:

Albizia odoratissima is a unique rare timber tree specie in South China. In order to carry out group genetics research on its germplasm resources, this study designed and developed EST-SSR markers of A. odoratissima based on the transcriptome sequencing results. In addition, A. procera, A. falcataria, Acacia melanoxylon, Erythrophloeum fordii and other related species were selected for analysis of interspecific generality. The results were as follows:(1)Among the 243 pairs of developed primers, 171 pairs could be successfully amplified to the target bands, and the effective amplification rates in Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii were 63.79%, 33.75%, 45.68%, 41.56% and 14.81%, respectively, and the polymorphism ratios in them were 23.87%, 12.20%, 9.01%, 3.96% and 2.78%, respectively. There were 18 pairs of primers commonly used among Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii.(2)There were 37 SSR polymorphism markers of Albizia odoratissima were obtained, ten polymorphism markers of A. procera and A. falcataria, four polymorphism markers of Acacia melanoxylon, and there was one polymorphism mark of Erythrophloeum fordii.(3)The developed EST-SSR markers can meet the needs of population genetic studies of Albizia odoratissima, and have good transferability and practicability in A. procera and A. falcataria and other related tree species. In conclusion, EST-SSR markers can provide a reliable research tool for genetic diversity evaluation of germplasm resources, fingerprint construction of breeding materials, and population mating system analysis of Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii. It is of great significance for the protection and utilization of Albizia odoratissima germplasm resource.