| 摘要: |
| 为探究葡萄CBF4基因的结构和表达特征,该研究以葡萄为材料,对葡萄 CBF4基因进行生物信息学及低温和硅酸钾响应分析。结果表明:(1)CBF4蛋白定位在细胞核,有5个磷酸化位点和14个糖基化位点,无信号肽,是一个亲水的、脂溶性较差的膜外蛋白。二级结构以无规则卷曲为主,比例为56.88%。该蛋白包含一个AP2/EREBP结构域。(2)CBF4蛋白的多序列和系统进化分析表明酿酒葡萄与美洲葡萄的同源性最高、亲缘关系最近。(3)荧光定量 PCR 分析显示,低温胁迫后CBF4基因在葡萄叶片中表达水平上调,说明CBF4基因可能参与了葡萄叶片低温胁迫的响应。低温条件下,施加硅酸钾CBF4基因表达具有差异性,说明该基因在不同的葡萄组织中对硅酸钾的响应机制可能不同。以上结果为进一步研究葡萄CBF4基因的功能和机理奠定了基础。 |
| 关键词: 葡萄, CBF4基因, 低温胁迫, 生物信息学, qRT-PCR |
| DOI：10.11931/guihaia.gxzw202008042 |
| 分类号:Q943 |
| 文章编号:1000-3142(2022)08-1433-08 |
| Fund project:甘肃省农牧厅生物技术专项(GNSW-2014-12); 国家自然科学基金(31560552, 51569002); 甘肃省自然科学基金(17JR5RA151)[Supported by Biotechnology Special Project of Gansu Provincial Department of Agriculture and Animal Husbandry(GNSW-2014-12); National Natural Science Foundation of China(31560552,51569002); Gansu Provincial Natural Science Foundation(17JR5RA151)]。 |
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| Bioinformatics of grape CBF4 gene and its response to low temperature and potassium silicate |
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ZHANG Hongmei, WANG Wangtian*, ZHANG Rui, YANG Ke,
WANG Baoqiang, WANG Cuiling
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College of Life Science and Technology, Gansu Agricultural University, Key Laboratory
of Crop Science in Arid Habitats, Lanzhou 730070, China
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| Abstract: |
| In order to explore the structure and expression characteristics of CBF4 gene from grapes, the study analyzed the grape CBF4 gene from the aspects of bioinformatics and low temperature and potassium silicate response. The results were as follows:(1)CBF4 protein was located in the nucleus, there were 5 phosphorylation sites and 14 glycation sites, without signal peptide. It was a hydrophilic, a poor lipid solubility and an extra-cellular protein. The secondary structure was dominated by random coil, with a ratio of 56.88%. The protein contained an AP2/EREBP domain.(2)The multiple sequence alignment and phylogenetic analysis of CBF4 protein showed that wine grapes and American grapes had the highest homology and the closest genetic relationship.(3)Quantitative real-time PCR analysis indicated that the expression level of CBF4 gene in grape leaves was up-regulated after low temperature stress, indicating that CBF4 gene may be involved in the response of grape leaves to low temperature stress. Under low temperature conditions, the CBF4 gene expression was different when potassium silicate was applied, indicating that the response mechanism of this gene to potassium silicate may be different in different grape tissues. These results lay a foundation for further study on the function and mechanism of CBF4 gene in grapes. |
| Key words: grape, CBF4 gene, low temperature stress, bioinformatics, qRT-PCR |
