罗汉果苷IIE在巨噬细胞糖尿病炎症模型中的作用

Mogroside IIE effects on macrophage in diabetic inflammatory models

关键词:  糖尿病炎症模型, 罗汉果苷IIE, 脂多糖, 棕榈酸, 小鼠巨噬细胞

Key words:  inflammation model of diabetes, mogroside IIE, lipopolysaccharide, palmitic acid, mouse macrophages

摘要:

为研究罗汉果苷IIE在脂多糖(LPS)和棕榈酸(PA)联合诱导小鼠巨噬细胞糖尿病炎症模型中的作用,该研究采用1 ng·μL^-1的LPS和100 μmol·L^-1的PA联合处理小鼠巨噬细胞RAW 264.7构建糖尿病炎症模型,并运用qRT-PCR分别检测0、1、3、6、12、24 h六个不同时间点细胞中炎症因子TNF-α mRNA的表达水平变化。结果表明:(1)1 ng·μL^-1的LPS和100 μmol·L^-1 的PA两者共同处理小鼠巨噬细胞RAW 264.7在24 h时产生的炎症协同作用最好;(2)在LPS(1 ng·μL^-1)和PA(100 μmol·μL^-1)联合诱导小鼠巨噬细胞12 h后,进而用20 μmol·L^-1的罗汉果苷IIE处理12 h,qRT-PCR检测结果发现,20 μmol·L^-1的罗汉果苷IIE处理12 h可显著降低LPS和PA联合诱导的细胞内炎症因子TNF-α mRNA表达水平,说明罗汉果苷IIE能够有效降低巨噬细胞的炎症水平。综上认为,罗汉果苷IIE可能在治疗糖尿病中起到一定的作用,该研究结果为糖尿病治疗新策略的提出提供了参考。

Abstract:

To investigate the role of mogroside IIE in the combination of lipopolysaccharide(LPS)and palmitic acid(PA)in the induction of diabetic inflammation in mouse macrophages, we used 1 ng· μl^-1 LPS and 100 μmol·l^-1 PA to establish a diabetic inflammation model of mouse macrophages RAW 264.7, and used qRT-PCR to detect the expression of inflammatory factor TNF-α mRNA in cells at 0, 1, 3, 6, 12, 24 h. The results were as follows:(1)The synergistic effect of LPS(1 ng· μL^-1)and PA(100 μmol· L^-1)on macrophage RAW 264.7 was the best at 24 h.(2)After mouse macrophages were induced with 1 ng·μL^-1 LPS and 100 μmol·L^-1 PA for 12 h, and treated with 20 μmol·L^-1 mogroside IIE for 12 h, qRT-PCR result showed that 20 μmol·L^-1 mogroside IIE treatment for 12 h could significantly reduce the expression of inflammatory factor TNF-α mRNA induced by LPS and PA, indicating that mogroside IIE can effectively reduce the inflammation level of macrophages. In conclusion, mogroside IIE may play a certain role in the treatment of diabetes, which provides a reference for the proposal of new strategies for the treatment of diabetes.