| 摘要: |
| 苯丙氨酸解氢酶(phenylalanine ammonia-lyase,PAL)基因家族参与苯丙烷类代谢过程,通过调控植物抗病次生物质的合成在植物抗逆反应中发挥重要作用。为明确谷子PAL基因家族在逆境胁迫下的表达规律,该研究利用生物信息学方法对谷子PAL基因家族进行鉴定和表达分析。结果表明:谷子具有11个PAL基因,在进化树中可分为3个亚家族,SiPAL7独自进化为一支。通过构建蛋白结构域发现PAL基因家族成员均含有保守的PAL结构域。启动子分析显示,PAL基因含有应答激素、逆境胁迫等多种因子的顺式作用元件,说明PAL基因广泛参与不同生物学调控过程。RT-qPCR结果显示,谷子PAL基因家族多为诱导型表达,不同光照条件下PAL基因表达量变化明显,不同基因具有不同响应模式,说明谷子PAL基因家族在参与光调节反应中发挥重要作用。谷子PAL基因高度保守,广泛响应不同非生物胁迫,具有表达特异性。该研究结果为揭示PAL基因家族在调节谷子抗性及胁迫应答过程中的作用提供了参考。 |
| 关键词: 谷子, PAL基因家族, 生物信息学分析, 蛋白结构, 表达分析 |
| DOI：10.11931/guihaia.gxzw202012060 |
| 分类号:Q943 |
| 文章编号:1000-3142(2022)09-1572-10 |
| Fund project:国家重点研发计划项目“十三五”国家粮食丰产增效科技创新专项(2017YFD0300305); 河北省科技厅项目(19226420D)[Supported by National Science and Technology Innovation Project of the 13th Five-Year Plan(2017YFD0300305); Hebei Science and Technology Department Project(19226420D)]。 |
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| Genome-wide identification and expression of millet PAL gene family |
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MENG Yaxuan, SUN Yingqi, ZHAO Xinyue, WANG Fengxia,
WENG Qiaoyun, LIU Yinghui*
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College of Agriculture and Forestry, Hebei North University, Zhangjiakou 075000, Hebei, China
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| Abstract: |
| Phenylalanine ammonia-lyase(PAL)involves in the process of phenylpropane metabolism, and PAL gene family plays an important role in the process of plant resistance through regulating the synthesis of plant antibiotics. In order to clarify the expression and the comprehensive analysis of the millet PAL gene family under adversity stress, bioinformatics methods were used to identify and analyze the PAL gene family in millet. The results were as follows: Millet had 11 PAL genes, which were unevenly distributed in different chromosomes. All the PAL gene family contained conserved PAL domains with one to six exons. Phylogenetic analyses showed the PAL gene could be divided into three subfamilies, except that SiPAL7 evolved into one by itself. PAL gene contained cis-acting elements that responded to hormones, adversity stress and other factors, indicating that PAL gene was widely involved in different biological regulation processes. RT-qPCR results showed that millet gene family were mainly inducible expressions, and the expression patterns of PAL gene changed obviously under different light conditions, and different genes had different response patterns, indicating that the millet PAL gene family played an important role in the light regulation response. The PAL gene of millet is highly conserved, responds to different abiotic stresses and has expression specificity. The results suggest that PAL genes may play important roles in the regulation of development and stress responses. |
| Key words: millet(Setaria italica), PAL gene family, bioinformatics analysis, protein structure, expression analysis |
