| 摘要: |
| 白花刺续断在中国西藏是一种常用的药用植物,但其叶绿体全基因组的相关研究较少。为揭示该物种叶绿体全基因组的基本特征并探讨其谱系遗传结构,该研究利用Illumina测序平台对来自5个野生居群的10个白花刺续断个体进行二代测序,经组装、注释,得到10条完整的叶绿体全基因组序列,并对它们的基因组特征和居群间的谱系进化关系进行了初步研究。结果表明:(1)白花刺续断的叶绿体全基因组大小为155 335～156 266 bp,共注释113个基因,包括72个蛋白编码基因、30个tRNA基因和4个rRNA基因,其叶绿体基因组的大小、结构、GC含量及基因组成等方面在种内高度保守。(2)基因组比较分析表明,白花刺续断变异较大的片段均位于单拷贝区,且IR边界未出现明显的扩张和收缩。(3)群体遗传分析发现,白花刺续断的野生居群具有明显的地理遗传结构,不同居群间在遗传距离与地理距离上具有一定的相关性。研究认为,白花刺续断叶绿体基因组在种内居群水平上比较保守,且叶绿体基因组可在居群水平上揭示物种的地理遗传结构。这为后续开展刺续断属物种群体遗传学和系统发育基因组学研究奠定了基础。 |
| 关键词: 白花刺续断, 叶绿体全基因组, 药用植物, 基因, 系统发育基因组学 |
| DOI：10.11931/guihaia.gxzw202106057 |
| 分类号:Q943 |
| 文章编号:1000-3142(2022)10-1750-12 |
| Fund project:国家自然科学基金(32060091); 云南省中青年学术和技术带头人后备人才项目(202105AC160063); 大理大学中药资源与民族药创新团队项目(ZKLX2019318); 云南省李剑专家工作站(202005AF150013)[Supported by National Natural Science Foundation of China(32060091); Reserve Talents Project for Young and Middle Aged Academic(202105AC160063); Dali University Chinese Medicine Resources and Ethnic Medicine Innovation Team Project(ZKLX2019318); Li Jian Expert Workstation of Yunnan Province(202005AF150013)]。 |
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| Analysis of complete chloroplast genome characteristics from wild populations of Acanthocalyx alba |
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ZHANG Qian1, ZHANG Dequan1,2*
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1. College of Pharmacy, Dali University, Dali 671000, Yunnan, China;2. Yunnan Key Laboratory of Screening and
Research on Anti-pathogenic Plant Resources from Western Yunnan, Dali 671000, Yunnan, China
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| Abstract: |
| Acanthocalyx alba is a common medicinal plant in Tibetan of China, and there are few reports on its whole chloroplast genome now. In order to reveal basic characteristics of the whole chloroplast genome of A. alba and explore its phylogeographic structure, ten individuals from five wild populations were sequenced by Illumina sequencing platform. After assembly and annotation, ten complete chloroplast genome sequences were obtained. Moreover, genome characteristics and phylogenetic relationships were explored. The results were as follows:(1)The complete chloroplast genomes of A. alba were 155 335-156 266 bp. A total of 113 genes were annotated, including 72 protein-coding genes, 30 tRNA genes and four rRNA genes. The chloroplast genome was highly conserved in species in terms of size, structure, GC content and gene composition.(2)Genomic comparative analysis showed that the fragments with large variation were located in the single copy region, and there was no obvious expansion and contraction at the IR boundaries.(3)Analysis of population genetics revealed rich genetic diversity in the wild populations and significant geographic structure, and there were certain relationships between genetic distances and geographic distances. This study lays the foundation for studies on population genetics and phylogenomics of species in Acanthocalyx. |
| Key words: Acanthocalyx alba, complete chloroplast genome, medicinal plant, gene, phylogenomics |
