环境变化对水稻osfh1突变体成蛋白家族表达的影响

Environment change effects on the expression level of Formins in rice osfh1 mutant

关键词:  成蛋白, OsFH1, 根毛, qRT-PCR, 水稻, 生物信息分析

Key words:  formin, OsFH1, root-hair, qRT-PCR, rice, bioinformatic analysis

摘要:

水稻成蛋白基因成员OsFH1在水稻根毛的生长发育中起着关键作用,这一过程受到环境因素的调控,当前的研究对环境因素如何与OsFH1互作调控水稻根毛的机制尚未阐明。为探索水稻成蛋白成员是否在环境因素介导的osfh1突变体根毛表型恢复中发挥作用,该研究使用1/2 MS液体培养液与1/2 MS固体培养基处理osfh1突变体,通过qRT-PCR技术分析成蛋白家族成员表达量,并对成蛋白家族进行生物信息学分析。结果表明:(1)与野生型相比,在液培中osfh1突变体主根根毛缺失,地上部分较短,侧根数量增加,在固体培养中osfh1突变体根毛缺失表型得到恢复。(2)与野生型相比,当osfh1突变体从液培到固培环境时,OsFH16表达量下降,OsFH17表达量上升,并且差异显著。(3)OsFH1、OsFH16、OsFH17都是第一类成蛋白亚家族成员,都具有生长素、赤霉素以及厌氧等与环境胁迫相关顺式作用元件,并且预测到OsFH1、OsFH16和OsFH17定位于质膜行使功能。(4)OsFHs在不同组织的表达模式分析表明,OsFH1在根部表达水平较高,而OsFH16、OsFH17在根部表达量相对较低。综上认为,由于OsFH16、OsFH17、OsFH1之间亲缘关系较高,调控模式相近且三者都可能在细胞质膜上行使功能,因此OsFH16、OsFH17可能参与环境因素与osfh1共同改变根毛表型这一过程。该研究结果为解析环境与osfh1基因共同调控水稻根毛发育机制奠定了一定理论基础,为探索植物成蛋白基因功能提出了新方向。

Abstract:

Oryza sativa formin homology 1(OsFH1)plays a crucial role in rice root-hair growth and development, and the short root-hair phenotype of osfh1 was changed by environmental factors. However, the mechanism of how environmental factors interact with OsFH1 to regulate rice root-hair phenotype is still unknown. To determine whether OsFHs function in the process of osfh1 mutant root-hair phenotype recovery. The expression of OsFHs was analyzed by qRT-PCR in the osfh1 mutant treated under 1/2 MS liquid culture and 1/2 MS solid culture. Furthermore, qRT-PCR results were compared with bioinformatics analysis results. The results were as follows:(1)When compared with the wild type, osfh1 primary root showed a no root-hair. The osfh1 mutant showed a shorter shoot and more lateral roots. However, the no root-hair phenotype of osfh1 recovered under 1/2 MS solid culture treatment.(2)The expression of OsFH16 decreased and the expression of OsFH17 increased in the osfh1 mutant from liquid culture treatment to solid culture treatment with signifcant differences.(3)OsFH1, OsFH16 and OsFH17 all belonged to the Type Ⅱ subfamily, and all had cis-acting elements related to environmental stress, such as auxin, gibberellin, and anaerobic. OsFH1, OsFH16, and OsFH17 may be located in the plasma membrane to perform functions.(4)Analysis of the tissue-specific expression pattern of OsFHs showed that OsFH1 was highly expressed in the roots, while OsFH16 and OsFH17 were lowly expressed in the roots. In conclusion, this study suggests that OsFH1, OsFH16, OsFH17 have conservative structures and similar regulatory modes, and all three may function on the cell plasma membrane, therefore, OsFH16, OsFH17 may be involved in the process that environmental factors and osfh1 together alter the root-hair phenotype. Overall, this study lays a theoretical foundation for the mechanism research of environmental factors and osfh1 gene co-regulation of rice root-hair development and proposes a new direction for exploring the function of plant formin genes.