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| 白菜CesA基因家族鉴定及表达模式分析 |
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马宇辰1, 赵玉梅1,2, 黄丹霖1, 张梦晴1, 吴晓宇1,2, 王 洁1,2 ,
陈 雨1, 黄家保1,2, 段巧红1,2*
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1. 山东农业大学 园艺科学与工程学院, 山东 泰安 271000;2. 作物生物学国家重点实验室, 山东 泰安 271000
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| 摘要: |
| 为探究CesA基因家族在白菜生长发育及纤维素合成过程中的作用机制,该文通过生物信息学的方法,以白菜的全基因组序列为研究区域,进行理化特征、基因结构、进化特征、保守基序及结构域、顺式作用元件和组织表达等鉴定分析。结果表明:(1)共鉴定出16个编码纤维素合成酶亚基的CesA基因,该家族成员所编码蛋白的理论等电点为4.76～9.12,相对分子量为17.76～122.67 kD,长度为153～1 089 aa。(2)15个基因不均匀地分布于白菜的7条染色体上,Bra036008定位于scaffold上。(3)大部分成员包含4～14个外显子,1～11个保守基序。(4)该家族具有保守的DDD-QXXRW 保守功能域。(5)该家族编码蛋白主要分布在质膜上,二级结构以无规则卷曲与α-螺旋为主,多数成员都含有CesA蛋白典型的N端、C端和跨膜区。(6)CesA基因在茎中表达量相对较高,其中Bra011865、Bra023952和Bra029874在茎、叶、花中显著表达。该研究结果为后续深入研究CesA基因功能以及白菜生长发育研究奠定了基础。 |
| 关键词: 纤维素合成酶, 白菜, CesA, 基因家族, 基因表达 |
| DOI：10.11931/guihaia.gxzw202107004 |
| 分类号:Q943.2 |
| 文章编号:1000-3142(2022)12-2021-11 |
| Fund project:山东省自然科学基金重点项目(ZR2020KC017)[Supported by Natural Science Foundation Shandong Province(ZR2020KC017)]。 |
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| Identification and expression analysis of CesA gene family in Brassica rapa var. glabra |
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MA Yuchen1, ZHAO Yumei1,2, HUANG Danlin1, ZHANG Mengqing1, WU Xiaoyu1,2,
WANG Jie1,2, CHEN Yu1, HUANG Jiabao1,2, DUAN Qiaohong1,2*
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1. College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271000, Shandong,
China;2. State Key Laboratory of Crop Biology, Tai'an 271000, Shandong, China
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| Abstract: |
| In order to explore the role of CesA gene family in cellulose synthesis and development of Brassica rapa var. glabra, we identified the members of CesA genes in B. rapa var. glabra genome via bioinformatic method and the physiochemical properties, gene structures, evolutionary characteristics, conserved inotifs and domains, cis-acting elements and tissue expressions were indentified and analysed. The results were as follows:(1)We identified 16 BrCesA genes from 10 B. rapa var. glabra chromasomes with isoelectric point ranged from 4.76 to 9.12, molecular weight ranged from 17.76 to 122.67 kD and amino acid length ranged from 153 to 1 089 aa.(2)With an exception of Bra036008, which located in scaffold, the rest of 15 BrCesA genes unevenly distributed in seven chromosomes.(3)Most of CesA contained 4 to 14 exons and 1 to 11 conserved motifs.(4)This family had a DDD-QXXRW conserved functional domain.(5)The coding proteins of this family were mainly distributed on the plasma membrane, and the secondary structure was mainly random coil and α-helix, and most members contained the typical N-terminus, C-terminus and transmembrane regions of CesA protein.(6)CesA gene was expressed in relatively high amounts in stems, and Bra011865, Bra023952 and Bra029874 were significantly expressed in stem,leaf and flowers. The results of this study provide a basis for further research on the function of CesA gene and the growth and development of Brassica rapa var. glabra. |
| Key words: cellulose synthase, Brassica rapa var. glabra, CesA, gene family, gene expression |
