| 摘要: |
| TIFY基因家族在茶树激素信号转导及其逆境胁迫具有十分重要的作用。该文利用生物信息学方法对茶树基因组中的TIFY家族进行了鉴定,分析其理化性质、系统进化、基因结构、染色体定位、启动子区顺式作用元件、组织表达模式,并通过RT-qPCR对部分TIFY家族成员进行了非生物胁迫。结果表明:(1)茶树TIFY基因家族成员19个(CsTIFY1～CsTIFY19),分属于4个蛋白亚家族TIFY、JAZ、ZML 和 PPD,且不均匀地分布在8 条染色体上,按照进化关系及结构特点可分为 7 组,每组内具有相似的基因结构与保守基序组成。(2)CsTIFYs基因的启动子区具有多种包含激素和非生物胁迫响应的顺式作用元件,通过实时荧光定量(RT-qPCR)分析其家族成员对在茉莉酸甲酯、盐(20%氯化钠)、冷(4 ℃)以及干旱(20% PEG-6000)处理下反应强烈,部分基因在根与顶芽中有较高的表达量。由此推测,TIFY基因家族可能在茶树激素信号调控、胁迫响应、生长和发育等多方面发挥功能作用。 |
| 关键词: 茶树, TIFY基因家族, 成员鉴定, 功能分析, 基因表达 |
| DOI：10.11931/guihaia.gxzw202103048 |
| 分类号:Q943 |
| 文章编号:1000-3142(2022)12-2044-12 |
| Fund project:贵州省优秀青年科技人才培养项目 [黔科合平台人才 [2019]5651]; 贵州大学实验室开放项目(SYSKF2021-005)[Supported by Guizhou Province Outstanding Young Scientific and Technological Talent Training Project [Qianke Heping Platform Talents [2019]5651]; Guizhou University Laboratory Open Project(SYSKF2021-005)]。 |
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| Genome identification of Camellia sinensis TIFY gene family and its expression analysis of abiotic stress |
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YAO Xinzhuan1, ZHANG Baohui2, CHEN Hufang2, LV Litang1,2*
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1. College of Tea Sciences, Guizhou University, Guiyang 550025, China;2. College of Life Sciences/Key Laboratory of
Plant Resources Conservation and Germplasm Innovation in Mountainous Regions (Ministry of Education),
Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China
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| Abstract: |
| TIFY gene family plays a very important role in Camellia sinensis hormone signal transduction and its adversity stress. Bioinformatics methods were employed to identify the TIFY family members in the C. sinensis genome in this study, and the physical and chemical properties, system evolution, gene structure, chromosomal location, the cis-acting elements of promoter region and tissue expression pattern were also analyzed, and the results of RT-qPCR experiments verified the hormone response and stress response characteristics of some members of the TIFY family. The results were as follows:(1)There were 19 TIFY gene family members(CSTIFY1-CSTIFY19)in C. sinensis, which belonged to four protein subfamilies of TIFY, JAZ, ZML and PPD, and distributed unevenly on eight chromosomes. According to evolutionary relationship and structural characteristics, TIFY genes could be divided into seven groups, and members of each group had similar gene structure and conserved motif.(2)The promoter region of the CsTIFYs gene contained a varieties of cis-acting elements in response to abiotic stress and hormones, the RT-qPCR experiments proved that its family members were highly responsive to methyl jasmonate, salt(20% NaCl), cold(4 ℃)and drought(20% polyethylene glycol 6000)treatments, and some genes were highly expressed during the development of roots and apical buds. Based on the above results, it is speculated that the TIFY gene family may play roles in C. sinensis hormone signal regulation, stress defense response and growth and development. |
| Key words: Camellia sinensis, TIFY gene family, members identification, functional analysis, gene expression |
