| 摘要: |
| 为探讨花榈木体胚发生过程中不同碳氮源处理对胚性愈伤组织诱导、发育和有机物积累的影响,并筛选出有利于花榈木体胚发生的碳氮源,优化体胚发生体系,该研究以成熟胚为外植体,通过单因素试验分析3种碳源、4种蔗糖浓度和6种氮源处理下胚性愈伤组织诱导、发育和有机物积累的差异。结果表明:(1)蔗糖中胚性愈伤组织诱导率显著高于葡萄糖和麦芽糖,但其体胚诱导率、体胚分化率、胚性愈伤组织可溶性糖、淀粉和可溶性蛋白含量差异不显著。(2)随着蔗糖浓度的升高,胚性愈伤组织、体细胞胚(体胚)诱导率、体胚分化率、胚性愈伤组织重量和可溶性蛋白含量呈先升高后降低的趋势,均以添加30 g·L-1蔗糖最高,而胚性愈伤组织可溶性糖和淀粉含量呈增加的趋势。(3)在6种氮源处理中,胚性愈伤组织诱导率以添加500 mg·L-1谷氨酰胺的处理最高,体胚诱导率则以添加谷氨酰胺和水解酪蛋白的处理较高,但不同氮源处理间体胚分化率无差异; 添加有机氮源的处理其胚性愈伤组织可溶性蛋白含量显著高于无氮源处理。总之,不同的碳氮源通过影响花榈木胚性愈伤组织的诱导、发育和有机物的积累,从而影响其体胚诱导率,但对体胚分化率影响不显著。初步认为30 g·L-1蔗糖和500 mg·L-1谷氨酰胺作为碳氮源可促进花榈木体胚发生诱导。 |
| 关键词: 花榈木, 体胚发生, 胚性愈伤组织, 碳氮源, 有机物 |
| DOI：10.11931/guihaia.gxzw202106004 |
| 分类号:Q945.1 |
| 文章编号:1000-3142(2022)12-2109-08 |
| Fund project:国家自然科学基金项目(31460193); 贵州省高层次创新人才培养计划项目(【2016】5661); 贵州省林业厅项目 [黔林科合(2010)重大 02] [Supported by National Natural Science Foundation of China(31460193); High Level Innovative Talents Training Program of Guizhou Province( [2016] 5661); Project of Guizhou Forestry Department [Qian Lin Ke He(2010)Major 02]。 |
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| Effects of carbon and nitrogen sources on the induction, development and organic matter accumulation of embryogenic callus in Ormosia henryi |
|
WU Gaoyin1,2, WEI Xiaoli 1,3*, WANG Xiao1,3, WEI Yi1,3
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1. College of Forestry, Guizhou University, Guiyang 550025, China;2. College of Life Sciences, Guizhou Normal University, Guiyang
550025, China;3. Institute for Forest Resources &4.Environment of Guizhou, Guiyang 550025, China
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| Abstract: |
| In order to study the effects of different carbon and nitrogen sources treatments on the embryogenic callus(EC)induction, development and organic matter accumulation during somatic embryogenesis process in Ormosia henryi, and to screen the carbon and nitrogen sources conducive to somatic embryogenesis and optimize somatic embryogenesis system in O. henryi. Mature embryos were used as explants in O. henryi, the differences of EC induction, development and organic matter accumulation under three kinds of carbon sources, four kinds of sucrose concentrations and six kinds of nitrogen sources treatments were analyzed by single factor experiment. The results were as follows:(1)EC induction rate in medium supplemented with sucrose was significantly higher than with glucose and maltose, while there was no significant difference in somatic embryo(SE)induction rate, SE differentiation rate and the contents of soluble sugar, starch and soluble protein with EC.(2)With the increase of sucrose concentration, EC, SE induction rate, SE differentiation rate, EC weight and soluble protein content first increased and then decreased, which was the highest in 30 g·L-1 sucrose, while the soluble sugar and starch contents of EC showed an increasing trend.(3)In six kinds of nitrogen sources treatments,EC induction rate was the highest in 500 mg·L-1 glutamine, SE induction rates were higher in glutamine and casein hydrolysate, but there were no differences in SE differentiation rates, the soluble protein content of EC in the treatment with organic nitrogen source was significantly higher than that in the treatment without organic nitrogen source. In summary, the different carbon and nitrogen sources affected the induction, development and the organic matter accumulation of EC, and affected SE induction rate, but there were no significant differences in the SE differentiation rate. The study suggests that 30 g·L-1 sucrose and 500 mg·L-1 glutamine as carbon and nitrogen sources can promote SE induction in O. henryi. |
| Key words: Ormosia henryi, somatic embryogenesis, embryogenic callus, carbon and nitrogen sources, organic matter |
