| 摘要: |
| 为明确莲种胚对不同温度胁迫的耐受能力,探索莲种胚细胞在逆境胁迫下的生理响应规律,该研究以成熟莲子为材料,从形态学、抗性生理和抗氧化相关基因表达定量3个层面进行了研究。结果表明:(1)莲子对于高温与超低温具有较好的胁迫耐受能力,经70 ℃高温与-196 ℃超低温处理后,其发芽率和种芽长相比对照组无显著变化; 80 ℃及以上的高温处理会使莲种胚吸水萌发迟缓,发芽率降低50%及以上,种芽萌发变慢。(2)高温处理后,莲种胚细胞中抗氧化酶活性随吸水萌发过程呈上升趋势,非酶促抗氧化剂含量下降,细胞膜脂过氧化程度逐渐减轻,质膜完整性有所恢复。(3)高温处理下,莲种胚氧化应激相关基因(DHN Rab18、Cu/Zn SOD、Mn SOD、POD41、POD73、CAT1、GR、APX)积极参与胁迫响应,出现不同程度的上调表达,100 ℃处理组中DHN Rab18、Cu/Zn SOD、POD41、GR与APX上调表达幅度较大。综上结果认为,莲种胚具有良好的高温与低温胁迫耐受性,在不同温度胁迫下抗氧化系统与抗逆保护类蛋白对维持莲种胚的细胞活力可能发挥重要保护作用。 |
| 关键词: 莲种胚, 高温, 形态指标, 逆境生理, 基因定量 |
| DOI：10.11931/guihaia.gxzw202107003 |
| 分类号:Q948.1 |
| 文章编号:1000-3142(2022)12-2128-10 |
| Fund project:国家自然科学基金(31971705); 上海市自然科学基金(21ZR1434200)[Supported by National Natural Science Foundation of China(31971705); Natural Science Foundation of Shanghai(21ZR1434200)] |
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| Stress physiology of lotus embryo under temperature stress |
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WANG Gongda1, CHU Yunxia2, XU Zheng1, ZHANG Di1*, ZHANG Yiying2
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1. College of Design, Shanghai Jiao Tong University, Shanghai 200240, China;2. Institute for Agri-food Standards
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| Abstract: |
| In order to reveal the tolerance of lotus seed embryo to different temperature stress and explore the physiological response of lotus seed embryo cells under stress, mature lotus seeds were used as materials to perform morphology, stress physiology and quantitative analysis of antioxidant related gene expression. The results were as follows:(1)Lotus seeds had a good tolerance to high temperature and ultra-low temperature stress. The germination percentage and germination length of lotus seeds had no significant differences between the 70 ℃, -196 ℃ and the control group; at 80 ℃ and above treatment condition, the germination percentage of lotus seeds decreased more than 50%, and the germination of seed bud was slowed down.(2)The lotus seeds were germinated after high temperature treatment, the activities of antioxidant enzymes in lotus seed embryo cells gradually increased, the content of non-enzymatic antioxidant and the degree of membrane lipid peroxidation were continuously decreased during the whole germination process, and the integrity of plosma membrane recovered.(3)The oxidative stress-related genes including DHN Rab18, Cu/Zn SOD, Mn SOD, POD41, POD73, CAT1, GR, APX were up-regulated response to high temperature treatment, indicating that these genes had positive responses to heat stress. Furthermore, the DHN Rab18, Cu/Zn SOD, POD41, GR and APX were up-regulated significantly in the 100 ℃ treatment group. In summary, lotus seed embryo has good tolerance to high temperature and low temperature stress, and the antioxidant system and stress-resistant protective proteins may play important roles in maintaining the cell viability of lotus seed embryo under different temperature stresses. |
| Key words: lotus seed embryo, high temperature, morphological index, stress physiology, gene quantification |
