细叶十大功劳叶中总生物碱大孔树脂纯化及抗氧化研究

Purification and antioxidant activity of total alkaloids from leaves of Mahonia fortunei by macroporous resin

关键词:  细叶十大功劳叶, 总生物碱, AB-8树脂, 纯化, 抗氧化

Key words:  Mahonia fortunei leaf, total alkaloids, AB-8 macroporous resin, purification, antioxidation

摘要:

为确定细叶十大功劳(Mahonia fortunei)叶中总生物碱大孔树脂分离纯化的最佳工艺条件及抗氧化活性,该研究通过比较6种大孔吸附树脂对总生物碱的静态吸附和解吸附效果,优选出最佳树脂并考察其动态纯化总生物碱的工艺条件,并采用DPPH法对纯化前后的总生物碱抗氧化性能进行评价。结果表明:(1)AB-8型大孔吸附树脂纯化效果最好,其最佳工艺条件为上样浓度50 mg·mL^-1(生药浓度)、上样量26 BV、上样液流速2 BV·h^-1; 吸附完成后,以3 BV水洗后再以4 BV 50%乙醇洗脱,在此条件下得到的总生物碱含量由13.33%提高到56.64%。(2)各样品对DPPH自由基的清除能力为对照品Vc(IC₅₀=10.39 μg·mL^-1)>总生物碱纯化品(IC₅₀=39.08 μg·mL^-1)>总生物碱粗品(IC₅₀=55.28 μg·mL^-1)。综上表明,AB-8型大孔吸附树脂可有效富集细叶十大功劳叶中总生物碱有效部位,细叶十大功劳叶中总生物碱具有一定的抗氧化活性。

Abstract:

In order to determine the optimum conditions and antioxidant activity of total alkaloids from leaves Mahonia fortunei by macroporous resins, the best resins were selected by comparing the static adsorption and desorption effects of six kinds of macroporous adsorption resins on total alkaloids. The optimum process conditions for the dynamic purification of total alkaloids were investigated, and the anti-oxidation performance of total alkaloids before and after purification was evaluated by DPPH method. The results were as follows:(1)AB-8 macroporous adsorption resins had the best purification effects. The optimum process conditions were crude herbal dose of 50 mg·mL^-1, sample volume of 26 BV, sample flow rate of 2 BV·h^-1, eluted successively with 3 BV water and 4 BV 50% ethanol. Under these optimized conditions, the content of total alkaloids was increased from 13.33% to 56.64%.(2)The total alkaloids had good scavenging effects on DPPH· before and after purification. The scavenging rate of DPPH· was Vc(IC₅₀=10.39 μg·mL^-1)> purified total alkaloids(IC₅₀=39.08 μg·mL^-1)> crude total alkaloids(IC₅₀=55.28 μg·mL^-1). AB-8 macroporous adsorption resin can effectively enrich the effective parts of total alkaloids from leaves of Mahonia fortunei, and the total alkaloids have certain antioxidant activities.