马铃薯低氮肥胁迫响应TCP转录因子的鉴定与分析

Identification and analysis of TCP transcription factors in response to low nitrogen fertilizer stress in Solanum tuberosum

关键词:  马铃薯, TCP转录因子, 氮肥胁迫, 转录组测序, 生物信息学分析

Key words:  Solanum tuberosum(potato), TCP transcription factors, nitrogen fertilizer stress, transcriptome sequencing, bioinformatics analysis

摘要:

TCP转录因子是植物特有的一类转录因子,参与植物生物学过程的多个方面。为研究马铃薯TCP转录因子在响应低氮肥胁迫中的作用,该研究以氮肥供应不足(0.05 mmol·L^-1)和氮肥供应充足(7.5 mmol·L^-1)条件下马铃薯的根和叶片构建4个转录组文库进行测序,并对差异表达的TCP转录因子进行分析。结果表明:(1)在4个转录组文库中共鉴定TCP转录因子24个,它们主要分布在2号、3号、6号染色体上。(2)经结构域分析显示,24个TCP 转录因子均具有典型的basic-Helix-Loop-Helix结构域。(3)经系统进化分析显示,马铃薯与拟南芥TCP蛋白可聚集在一起,分属于10个亚类。(4)转录组测序结果显示,在低氮肥胁迫下,大多数TCP转录因子被抑制表达,有3个TCP转录因子在根中显著性差异表达,5个TCP转录因子在叶中特异性表达。(5)根据GO功能注释分析和马铃薯TCP转录因子与拟南芥TCP转录因子的亲缘关系分析推测,这些TCP转录因子参与了马铃薯对低氮肥胁迫的响应。该研究结果为进一步研究马铃薯与其他粮食作物TCP转录因子响应低氮肥胁迫的分子功能奠定了基础。

Abstract:

TCP transcription factors are a type of plant-specific transcription factors, which have been implicated in multiple aspects of plant biological processes. To study the role of potato TCP transcription factors in response to low nitrogen fertilizer stress, here, four transcriptome libraries from potato roots and leaves under the conditions of low nitrogen fertilizer(0.05 mmol·L^-1)and sufficient nitrogen fertilizer(7.5 mmol·L^-1)were constructed for transcriptome sequencing, respectively. Meanwhile, the differentially expressed TCP transcription factor were analyzed. The results were as follows:(1)A total of 24 TCP transcription factors were identified in the four transcriptome libraries, and mainly distributed on the second, third and sixth chromosomes.(2)The analysis of domains showed that all of 24 transcription factors contained basic-Helix-Loop-Helix domain.(3)The phylogenetic analysis showed that the TCP proteins of potato and Arabidopsis thaliana were closely related, and clustered into 10 subgroups.(4)Transcriptome sequencing results showed that the expression levels of most potato TCP transcription factors were suppressed by low nitrogen fertilizer stress. Among them, three TCP transcription factors were significantly differentially expressed in roots, while five TCP transcription factors were specifically expressed in leaves.(5)According to the GO functional analysis and the relationship between the potato and the A. thaliana TCP transcription factors, it is predicted that these TCP transcription factors were involved in response of potato to low nitrogen fertilizer stress. The research provides a foundation for further study on the molecular role of transcription factors in response to low nitrogen fertilizer stress in potato and other crops.