不同亚硒酸钠浓度对党参生长、生理特性和品质的影响

Effects of different selenium concentrations on growth, physiological characteristics and quality of Codonopsis pilosula

关键词:  施硒效应, 富硒党参, 党参炔苷, 胁迫反应, 双重效应

Key words:  effect of Se supplementation, Se-enriched Codonopsis pilosula, lobetyolin, stress response, double effect

摘要:

为综合了解党参的施硒效应,该研究基于水培试验探究不同亚硒酸钠浓度处理条件下党参幼苗的硒积累、生长、生理和品质的变化规律。结果表明:(1)适宜硒浓度(0.2 mg·L^-1)可以促进党参的叶片面积、株高、生物量的增长,而高硒浓度(10 mg·L^-1)则抑制党参生长。(2)增加亚硒酸钠浓度和硒暴露时间均可提高党参幼苗在根和叶中的硒含量,不同器官硒积累表现为根>叶>茎。(3)适宜硒浓度(0.2 mg·L^-1)处理可提高光合色素含量和根系活力,并减少丙二醛、脯氨酸和过氧化氢的积累,而高硒浓度(10 mg·L^-1)则与之相反。(4)适宜硒浓度(0.2 mg·L^-1)有利于党参炔苷、多糖、可溶性蛋白的积累,而高硒浓度(10 mg·L^-1)可以对多糖和可溶性蛋白的积累产生不利影响。综上认为,亚硒酸钠对党参具有双重效应,施加适量浓度对党参的生长、生理和品质有益,并以0.2 mg·L^-1硒浓度处理的效果最佳。该研究结果有助于了解亚硒酸钠浓度对施硒效应的影响,为富硒党参的基础研究及栽培提供了理论依据。

Abstract:

To comprehensively understand the effect of selenium(Se)supplementation on Codonopsis pilosula, effects of sodium selenite levels on Se accumulation, growth, physiology and quality were explored by using hydroponic experiments. The results were as follows:(1)Treatment with 0.2 mg·L^-1 Se promoted leaf size, plant height and biomass of C. pilosula, while treatment with 10 mg·L^-1 Se suppressed them.(2)Both increasing Se level and exposure time to C. pilosula seedlings could raise its Se contents in root and leaf, and Se was distributed in organs with an order of root>leaf>stem.(3)Treatment with 0.2 mg·L^-1 Se increased photosynthetic pigment content and root activity, and reduced malondialdehyde, proline and hydrogen peroxide contents, while treatment with 10 mg·L^-1 Se had opposite effects.(4)Treatment with 0.2 mg·L^-1 Se had positive effects on accumulations of lobetyolin, polysaccharide, and soluble protein, while treatment with 10 mg·L^-1 Se had negative effects on the accumulations of polysaccharide and soluble protein. In summary, sodium selenite has double effect on C. pilosula; application of appropriate Se is beneficial to its growth, plant physiology and quality, and 0.2 mg·L^-1 Se level is the optimum. This study may improve us to understand the effect of selenite level on the biology effect of C. pilosula, which will be useful for the basic research and cultivation of Se-enriched C. pilosula.