全缘叶紫珠化学成分研究

Chemical constituents of Callicarpa integerrima

关键词:  紫珠属, 全缘叶紫珠, 化学成分, 苯乙醇苷类, 三萜类

Key words:  Callicarpa, Callicarpa integerrima, chemical constituents, phenylethanoids, triterpenoids

摘要:

全缘叶紫珠(Callicarpa integerrima)具有祛风散结和治风湿瘰疬的功效,但目前对其化学成分的报道较少。为探究全缘叶紫珠根、茎的化学成分,该研究利用硅胶柱层析色谱、Sephadex LH-20葡聚糖凝胶柱层析色谱、ODS反相硅胶柱层析色谱以及高效液相色谱等现代分离方法对全缘叶紫珠根和茎的95%乙醇提取物进行系统的分离纯化,并运用NMR和ESI-MS等现代波谱技术对化合物进行结构鉴定。结果表明:从全缘叶紫珠根和茎的95%乙醇提取物中共鉴定了15个化合物分别为豆甾烷-4-烯-3-酮(1)、(24R)-5α-豆甾烷-3,6-二酮(2)、2'-羟基-4'-甲氧基二氢查尔酮(3)、α-香树脂醇(4)、 β-谷甾醇(5)、熊果酸(6)、对羟基间甲氧基苯甲酸(7)、4-羟基吡啶(8)、对羟基苯甲酸(9)、连翘酯苷B(10)、nepetifosides D(11)、异毛蕊花苷(12)、毛蕊花苷(13)、pedicularioside M(14)、 β-甲氧基连翘酯苷B(15)。除化合物4-6、12和13外,其他化合物均为首次从全缘叶紫珠植物中分离得到,其中化合物1、2、3、8、11和14为首次从紫珠属植物中分离得到。该研究结果丰富了全缘叶紫珠植物的化合物库,为该药用植物的进一步开发利用奠定了科学基础。

Abstract:

Callicarpa integerrima has very good effect in emoving blood stasis and resolving static blood and treatment of rheumatism evil. However, there are few reports on its chemical constituents. In order to explore the chemical constituents from the roots and stems of C. integerrima, the 95% ethanol extracts of C. integerrima roots and stems were isolated and purified by diverse column chromatography, such as silica gel, Sephadex LH-20 gel column, ODS column chromatography, and preparative HPLC. In addition, these compounds were identified on the basis of NMR, ESI-MS as well as other modern spectral techniques. The results were as follows: A total of 15 compounds were isolated from the 95% ethanol extracts of the roots and stems of C. integerrima, which were identified as stigmast-4-en-3-one(1),(24R)-5α-stigmastane-3,6-dione(2), 2'-hydroxy-4'-methoxydihydrochalcone(3), α-amyrin(4), β-sitosterol(5), ursolic acid(6), 4-hydroxy-3-methoxy-benzoic acid(7), 4-pyridinol(8), p-hydroxybenzoic acid(9), forsythoside B(10), nepetifosides D(11), isoacteoside(12), acteoside(13), pedicularioside M(14), β-methoxy forsythoside B(15). All compounds, except for compounds 4-6, 12 and 13, were isolated from C. integerrima for the first time. Furthermore, compounds 1, 2, 3, 8, 11 and 14 were isolated from the genus of Callicarpa for the first time. Thus, the results of this research has enriched the compound library and afford a scientific foundation for the further rational use of C. integerrima.