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| 古树大理茶优势内生真菌1个新四氢-β-咔啉二酮哌嗪的分离鉴定 |
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程璐瑶1, 陈肖学1, 张颖君2, 黄永林3, 朱国磊1, 李 娜2, 赵 平1*
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1. 西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室, 昆明 650224;2. 中国科学院昆明植物研究所
植物化学与西部植物资源持续利用国家重点实验室, 昆明 650201;3. 广西壮族自治区
中国科学院 广西植物研究所,
广西植物功能物质研究与利用重点实验室, 广西 桂林 541006
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| 摘要: |
| 为挖掘古树大理茶优势内生真菌间座壳属菌株Diaporthe tectonigena的化学成分,该研究采用硅胶、大孔吸附树脂Diaion HP20、葡聚糖凝胶LH-20等柱层析方法,对该菌株的大米固态发酵提取物进行分离纯化,并通过HRMS、1H-NMR、13C-NMR、HSQC、HMBC和COSY等波谱分析,对所得化合物进行结构鉴定。结果表明:(1)从该菌株大米固态发酵提取物中分离得到4个化合物,其中新化合物1鉴定为四氢-β-咔啉二酮哌嗪类生物碱,命名为tectonicgenazine A。(2)3个已知化合物分别鉴定为trans-cyclo-(D-tryptophanyl-L-tyrosyl)(2)、1H-吲哚-3-羧酸-2,3-二羟基丙酯(3)和N-羟乙基-2-乙酰基吡咯(4),其中化合物3为首次从自然界中分离所得。 |
| 关键词: 大理茶, 内生真菌, 间座壳属, 固态发酵, 二酮哌嗪 |
| DOI：10.11931/guihaia.gxzw202206008 |
| 分类号:Q946 |
| 文章编号:1000-3142(2023)07-1252-06 |
| Fund project:国家自然科学基金(32060107, 32060327); 云南省教育厅科学研究基金(2021Y215)。 |
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| Isolation and identification of a new tetrahydro-β-carboline diketopiperazine from a predominant endophytic fungus in ancient Camellia taliensis |
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CHENG Luyao1, CHEN Xiaoxue1, ZHANG Yingjun2, HUANG Yonglin3,
ZHU Guolei1, LI Na2, ZHAO Ping1*
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1. Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest
China, Southwest Forestry University, Kunming 650224, China;2. State Key Laboratory of Phytochemistry and Plant Resources in West
China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;3. Guangxi Key Laboratory of
Functional PhytochemicalsResearch and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous
Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
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| Abstract: |
| To investigate the chemical constituents of Diaporthe tectonigena, a predominant endophytic fungus in the ancient tea tree of Camellia taliensis, the rice solid-state fermentation extract was isolated and purified by silica gel, Diaion HP20 and Sephadex LH-20 column chromatographies, and the chemical structures were elucidated by extensive HRMS, 1H-NMR, 13C-NMR, HSQC, HMBC and COSY spectroscopic analyses. The results were as follows:(1)Four compounds are isolated from the rice solid-state fermentation extract of Diaporthe tectonigena, including a new tetrahydro-β-carboline diketopiperazine alkaloid named tectonicgenazine A(1).(2)Three known compounds, trans-cyclo-(D-tryptophanyl-L-tyrosyl)(2), 1H-indole-3-carboxylic acid-2,3-dihydroxypropyl ester(3)and N-hydroxyethyl-2-acetylpyrrole(4)were obtained and identified, and Compound 3 was isolated from nature for the first time. |
| Key words: Camellia taliensis, endophytic fungus, Diaporthe, solid-state fermentation, diketopiperazine |
