| 摘要: |
| 该研究采用TLC方法,对不同产地大罗伞树进行定性鉴别,并采用HPLC方法对大罗伞树不同产地和不同部位的岩白菜素进行了含量测定。其中,色谱柱为SinoChrom ODS-BP C18(250 mm × 4.6,5 μm),流动相为甲醇-水(23:77),流速为1.0 mL·min-1,检测波长为275 nm。结果表明:10批不同产地大罗伞树药材的TLC均可检出岩白菜素斑点; 10批不同产地大罗伞树药材中岩白菜素平均含量为1.67%,但产地间含量差异较大,最高含量可达2.58%,最低含量0.95%; 大罗伞树不同部位中岩白菜素含量有所差异,地下部分(根及根茎)含量较高,平均值为4.05%,地上部分(茎枝和叶)含量较低,平均值为1.21%。所建立的大罗伞树药材TLC鉴别和岩白菜素含量测定方法操作简便、专属性强、重现性好、结果准确。该研究结果能准确鉴别大罗伞树药材真伪,并测定药材中岩白菜素的含量,为大罗伞树药材质量标准的建立提供了依据,对于控制壮药大罗伞树药材质量具有重要意义。 |
| 关键词: 大罗伞树, 岩白菜素, TLC, HPLC |
| DOI：10.11931/guihaia.gxzw201605002 |
| 分类号:Q946 |
| 文章编号:1000-3142(2016)02-0260-05 |
| 基金项目:广西科技厅重大专项(桂科重1355001-4-2); 广西壮药质量标准(第三卷)质量评价与标准研究项目(GXZC2014-G3-1577-YLZB-B-2); 广西卫生厅中医药民族医药科研课题(CZZC13-13)[Supported by the Key Programof Guangxi Scientific and Technological Office(1355001-4-2); Quality Standards of Guangxi Zhuang Medicine(Vol.Ⅲ)Quality Evaluation and Standards Research Program(GXZC2014-G3-1577-YLZB-B-2); Traditional Chinese Medicine Research Program of Guangxi Health Department(CZZC13-13)]。 |
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| Qualitative and quantitative analysis in Zhuang medicine Ardisia quinquegona from different habitats and parts |
|
ZHANG Zhen1, LI Zheng-Wen1, HUANG Rui-Song2*
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1. College of Agriculture, Guangxi University, Nanning 530004, China;2. Guangxi Academy of
Minority Nationality Medicine and Pharmacology, Nanning 530001, China
1. College of Agriculture, Guangxi University, Nanning 530004, China; 2. Guangxi Academy of
Minority Nationality Medicine and Pharmacology, Nanning 530001, China
|
| Abstract: |
| We indentified with TLC and analyzed the contents of Bergenin through different regions and parts of the Ardisia quinquegona to provide experimental basis for the Zhuang medicine quality evaluation and standard. The different habitats of A. quinquegona were identified by TLC. The contents of Bergenin in different habitats and parts of A. quinquegona were determinated by HPLC. Using SinoChrom ODS-BP C18 chromatographic column(250 mm × 4.6, 5 μm)with the detection wavelength of 275 nm, methanol-water(23:77)as mobile phase with the flow rate of 1.0 mL·min-1. The results showed that ten batches different eares of A. quinquegona which contained Bergenin by TLC. And the average content of bergenin was 1.67%, but there were significant differences among them, the highest content up to 2.58%, the lowest content 0.95%. The contents of A. quinquegona of different parts were different, the underground part(root and rhizome)contents higher with an average of 4.05%, the ground parts(stems and leaves)were lower with an average of 1.21%. TLC identification method and determination of Bergenin in A. quinquegona were simple, reproductive and accurat. The chemical method could accurately identify the authenticity of A. quinquegona and determination in Bergenin. This study provides experimental basis for the establishment of A. quinquegona medicinal Zhuang medicine quality standards for the control of its quality is of great significance. |
| Key words: Ardisia quinquegona, Bergenin, TLC, HPLC |
